Elevated Interleukin-21 Correlated to Th17 and Th1 Cells in Patients with Immune Thrombocytopenia
Elevated Interleukin-21 Correlated to Th17 and Th1 Cells in Patients with Immune Thrombocytopenia
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免疫性血小板减少症患者中白细胞介素 21 升高与 Th17 和 Th1 细胞相关
DOI：
10.1007/s10875-009-9353-1
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发表时间：
2010-03-01
影响因子：
9.1
通讯作者：
Hou, Ming
中科院分区：
文献类型：
--
作者：
Zhu, Xiaojuan;Ma, Daoxin;Hou, Ming
白细胞介素-21（IL-21）在自身免疫性疾病的发展中至关重要。IL-21在免疫性血小板减少症（ITP）发病机制中的作用尚不清楚，我们采用酶联免疫吸附试验和流式细胞术检测了ITP患者和对照组中IL-21、IL-17和IFN-γ的表达。用改良的单克隆抗体特异性固定血小板抗原法检测抗血小板GPIIb/IIIa和/或GPIb/IX自身抗体，流式细胞术检测CD 3（+）CD 8（-）T细胞和CD 3（+）CD 8（+）T细胞上IL-21的表达。ITP患者血浆IL-21水平、CD 3（+）CD 8（-）IL-21（+）T细胞和CD 3（+）CD 8（+）IL-21（+）T细胞百分比均显著高于对照组。ITP患者的CD 3（+）CD 8（-）IL-17（+）T（Th 17）、CD 3（+）CD 8（-）IFN-γ（+）T（Th 1）和CD 3（+）CD 8（+）IFN-γ（+）T（Tc 1）细胞百分比也显著增加。此外，我们发现CD 3（+）CD 8（-）IL-21（+）T细胞和Th 17细胞之间存在显著的正相关性。IL-21在ITP患者中的作用可能与Th 17和Th 1细胞有关，阻断IL-21可能是治疗ITP尤其是活动期ITP的一种合理的策略。
Interleukin-21 (IL-21) is critical in the development of autoimmune diseases. The role of IL-21 in the pathogenesis of immune thrombocytopenia (ITP) remains unknown.We examined the expression of IL-21, IL-17, and interferon (IFN)-gamma in ITP patients and controls by enzyme-linked immunosorbent assay and flow cytometry. Detection of specific anti-platelet GPIIb/IIIa and/or GPIb/IX autoantibodies was measured by modified monoclonal antibody specific immobilization of platelet antigens.IL-21 was expressed on both CD3(+)CD8(-) T cells and CD3(+)CD8(+) T cells by flow cytometry. Plasma IL-21 level and the percentage of CD3(+)CD8(-)IL-21(+) T cells and CD3(+)CD8(+)IL-21(+) T cells were significantly elevated in ITP patients compared to controls. The percentage of CD3(+)CD8(-)IL-17(+) T (Th17), CD3(+)CD8(-)IFN-gamma(+) T (Th1), and CD3(+)CD8(+)IFN-gamma(+) T (Tc1) cells also significantly increased in ITP patients. Moreover, we found a significant positive correlation between CD3(+)CD8(-)IL-21(+) T cells and Th17 cells. In addition, a positive correlation between CD3(+)CD8(-)IL-21(+) T cells and Th1 cells was also found.Together, our results indicated a possible role of IL-21 in ITP patients correlated to Th17 and Th1 cells, and blockade of IL-21 may be a reasonable therapeutic strategy for ITP especially those with active disease.