Studies of factors related to the differentiation of alkaline phosphatases derived from several tissues.
Studies of factors related to the differentiation of alkaline phosphatases derived from several tissues.
复制标题
研究与多种组织来源的碱性磷酸酶分化相关的因素。
DOI：
10.1016/0003-9861(61)90179-5
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发表时间：
1961
影响因子：
3.9
通讯作者：
M. Schlamowitz
中科院分区：
文献类型：
--
作者：
W. Landau;M. Schlamowitz
肝、肾、脾和骨的碱性磷酸酶与肠酶的区别在于这些酶对几种磷酸单酯底物的相对活性，这些酶在pH9.7时与β-甘油磷酸的Km值没有检测到这些差异。来自抑制研究和热灭活研究的证据表明，对各种磷酸单酯酶的活性是由于一种酶具有广泛的特异性，而不是一个家庭的单独的酶。本文还讨论了酶制剂方式对磷酸酶色谱行为的影响以及这一发现对酶变体的意义。
The alkaline phosphatases of liver, kidney, spleen, and bone were differentiated from the intestinal enzyme by the relative activities of these enzymes on several phosphomonoester substrates.Kmvalues for these enzymes with β-glycerophosphate at pH 9.7 failed to detect these differences. Evidence derived from inhibition studies and from studies on heat inactivation indicate that the activity of the enzyme against a variety of phosphomonoesters is due to an enzyme with a broad specificity rather than a family of separate enzymes. The alteration of chromatographic behavior of the phosphatases by the mode of enzyme preparation and the significance of this finding to enzyme variants is discussed.