Challenges in paper-based fluorogenic optical sensing with smartphones.
Challenges in paper-based fluorogenic optical sensing with smartphones.
复制标题
DOI：
10.1186/s40580-018-0146-1
复制
发表时间：
2018
期刊：
影响因子：
11.7
通讯作者：
Yoon JY
中科院分区：
文献类型：
--
作者：
Ulep TH;Yoon JY
光学优越、可调谐的荧光纳米技术的应用早已在许多化学和生物传感应用中得到证明。与微流体技术相结合，即在芯片实验室平台上，这种荧光纳米技术通常具有极高的灵敏度，有时可以达到单分子水平。近年来，人们对将荧光纳米技术转化为基于纸的化学和生物传感平台，作为传统硅基微流体基板的简单、低成本、一次性替代品的兴趣达到了峰值。另一方面，智能手机作为光学检测系统以及用户界面和数据处理组件的集成已经被广泛尝试，作为车载定量处理、增强移动性和与信息网络互连的门户。智能手机传感可以集成到这些基于纸张的荧光分析中，以展示极高的灵敏度以及易用性和低成本。然而，对于这些新兴技术，总有一些必须解决的技术限制；例如，纸张的自身荧光会干扰荧光传感；智能手机闪光灯在荧光激发方面的局限性智能手机摄像头在检测窄带荧光发射等方面的局限性。在这篇综述中，物理光学装置、数字增强算法和各种荧光测量技术被讨论并确定为进一步改进基于纸张的智能手机荧光光学传感的机会领域。
Application of optically superior, tunable fluorescent nanotechnologies have long been demonstrated throughout many chemical and biological sensing applications. Combined with microfluidics technologies, i.e. on lab-on-a-chip platforms, such fluorescent nanotechnologies have often enabled extreme sensitivity, sometimes down to single molecule level. Within recent years there has been a peak interest in translating fluorescent nanotechnology onto paper-based platforms for chemical and biological sensing, as a simple, low-cost, disposable alternative to conventional silicone-based microfluidic substrates. On the other hand, smartphone integration as an optical detection system as well as user interface and data processing component has been widely attempted, serving as a gateway to on-board quantitative processing, enhanced mobility, and interconnectivity with informational networks. Smartphone sensing can be integrated to these paper-based fluorogenic assays towards demonstrating extreme sensitivity as well as ease-of-use and low-cost. However, with these emerging technologies there are always technical limitations that must be addressed; for example, paper’s autofluorescence that perturbs fluorogenic sensing; smartphone flash’s limitations in fluorescent excitation; smartphone camera’s limitations in detecting narrow-band fluorescent emission, etc. In this review, physical optical setups, digital enhancement algorithms, and various fluorescent measurement techniques are discussed and pinpointed as areas of opportunities to further improve paper-based fluorogenic optical sensing with smartphones.