Intervening sequences in rrl genes and fragmentation of 23S rRNA in genera of the family Enterobacteriaceae
Intervening sequences in rrl genes and fragmentation of 23S rRNA in genera of the family Enterobacteriaceae
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DOI：
10.1128/jb.183.19.5782-5787.2001
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发表时间：
2001-10-01
影响因子：
3.2
通讯作者：
Sanderson, KE
中科院分区：
文献类型：
--
作者：
Pronk, LM;Sanderson, KE
23S rRNA 的 rrl 基因中的插入序列 (IVS) 被转录，但随后被 RNase III 去除，而无需在 RNA 加工过程中重新连接，从而产生片段化的 rRNA。我们使用 PCR 检查了大约 240 种肠杆菌科菌株中是否存在 IVS。在属于埃希氏菌属、志贺氏菌属、肠杆菌属、欧文氏菌属、尤文氏菌属、哈夫氏菌属、克鲁维拉属、摩根氏菌属、泛菌属或沙雷氏菌属的菌株中未检测到 IVS。在产酸克雷伯菌、无丙酸柠檬酸杆菌和斯氏普罗维登斯菌中检测到了以前未报告的 IVS；先前报道的 IVS 存在于沙门氏菌、变形杆菌、普罗维登斯菌和耶尔森氏菌中。 IVS 的零星分布表明 IVS 的横向遗传转移。
Intervening sequences (IVSs) in the rrl genes for 23S rRNA are transcribed but later removed by RNase III without religation during RNA processing, leading to fragmented rRNA. We examined about 240 strains of the family Enterobacteriaceae for presence of IVSs using PCR. No IVSs were detected in strains belonging to Escherichia, Shigella, Enterobacter, Erwinia, Ewingella, Hafnia, Kluyvera, Morganella, Pantoea, or Serratia. Previously unreported IVSs were detected in Klebsiella oxytoca, Citrobacter amalonaticus, and Providencia stuartii; previously reported IVSs are in species of Salmonella, Proteus, Providencia, and Yersinia. The sporadic distribution of IVSs indicates lateral genetic transfer of IVSs.