Preparation of rabbit anti-Ganoderma sinensis immunomodulatory protein polyclonal antibody
Preparation of rabbit anti-Ganoderma sinensis immunomodulatory protein polyclonal antibody
复制标题
DOI：
10.5897/ajmr10.471
复制
发表时间：
2011-06
影响因子：
--
通讯作者：
Li Qizhang;Zheng Shengbo;W. Xuefei;Bao Tingwen;Zhou Xuanwei
中科院分区：
文献类型：
--
作者：
Li Qizhang;Zheng Shengbo;W. Xuefei;Bao Tingwen;Zhou Xuanwei
灵芝免疫调节蛋白(FIP-GSI)是真菌免疫调节蛋白(FIP)家族中的一种新蛋白。以原核表达的重组FIP-GSI为基础，免疫新西兰大白兔，制备抗FIP-GSI多克隆抗体。用ELISA法和Western印迹法检测多克隆抗体的效价。结果表明，成功制备了效价高、特异性强的抗FIP-GSI多克隆抗体，间接ELISA检测效价为1：625,000，Western印迹法检测到特异性条带。本研究建立了免疫印迹法鉴定FIP-GSI的方法，为进一步探讨FIP-GSI的免疫功能奠定了基础。关键词：灵芝真菌免疫调节蛋白(FIP-GSI)、多克隆抗体、酶联免疫吸附试验、免疫印迹。
Ganoderma sinensis immunomodulatory protein (FIP-gsi) was a new protein in fungal immunomodulatory protein (FIP) family. Based on the recombinant FIP-gsi expressed inEscherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its efficient value was 1:625,000 detected by indirect ELISA, and a special band had been observed by Western blot method. This study established a method to identify FIP-gsi by immunoblotting, and will lay a foundation for further exploring the immunologic function of FIP-gsi. Key words: Ganoderma sinensis fugal immunomodulatory proteins (FIP-gsi), polyclonal antibody, ELISA, Western blot.