Somatostatin (SSTR2) receptors mediate phospholipase C-independent Ca2+ mobilization in rat AR42J pancreas cells.
Somatostatin (SSTR2) receptors mediate phospholipase C-independent Ca2+ mobilization in rat AR42J pancreas cells.
复制标题
生长抑素 (SSTR2) 受体介导大鼠 AR42J 胰腺细胞中不依赖磷脂酶 C 的 Ca2 动员。
DOI：
10.1006/bbrc.1995.2259
复制
发表时间：
1995
影响因子：
3.1
通讯作者：
J. Taylor
中科院分区：
文献类型：
--
作者：
J. Taylor
表达生长抑素-SSTR 2型受体的大鼠AR 42 J胰腺细胞对SSTR 2选择性生长抑素（SRIF）激动剂配体的反应是细胞内Ca 2+的剂量依赖性增加。除了SRIF-14和SRIF-28之外，最有效的SRIF肽是环状八肽BIM-23014 C、BIM-23023、SMS 201-995和环状六肽MK-678和BIM-23027。SSTR 3和SSTR 5选择性配体BIM-23056和BIM-23052分别是无活性的和弱活性的。没有SRIF肽刺激磷酸肌醇周转，表明Ca 2+动员是独立的磷脂酶C激活。在无钙培养基中孵育取消了细胞内Ca 2+的增加。这些结果表明，AR 42 J细胞中SSTR 2受体的激活打开了细胞表面钙通道。
Rat AR42J pancreas cells, which express somatostatin-SSTR2 type receptors, responded to SSTR2-selective somatostatin (SRIF) agonist ligands with a dose-dependent increase in intracellular Ca2+. In addition to SRIF-14 and SRIF-28, the most potent SRIF peptides were the cyclic octapeptides, BIM-23014C, BIM-23023, SMS 201-995, and the cyclic hexapeptides, MK-678 and BIM-23027. The SSTR3 and SSTR5-selective ligands, BIM-23056 and BIM-23052, were inactive and weakly active, respectively. None of the SRIF peptides stimulated inositol phosphate turnover, indicating that Ca2+ mobilization was independent of phospholipase C activation. Incubation in calcium-free medium abolished the increase in intracellular Ca2+. These results indicate that activation of SSTR2 receptors in AR42J cells opens cell-surface calcium channels.