SENSITIVE TO SALT1, An Endoplasmic Reticulum-Localized Chaperone, Positively Regulates Salt Resistance
SENSITIVE TO SALT1, An Endoplasmic Reticulum-Localized Chaperone, Positively Regulates Salt Resistance
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对盐1敏感，一种内质网定位伴侣，积极调节耐盐性
DOI：
10.1104/pp.18.00840
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发表时间：
2018-11-01
期刊：
影响因子：
7.4
通讯作者：
Zheng, Chengchao
中科院分区：
文献类型：
--
作者：
Guan, Peiyan;Wang, Jun;Zheng, Chengchao
盐胁迫严重影响植物的生长发育。通过遗传筛选，我们鉴定了一个对Scan敏感的拟南芥突变体(Ses1)，并对其进行了鉴定。在盐胁迫下，SES1基因普遍表达并诱导表达。Ses1对盐敏感的表型与Na的过度积累无关。也不是对耐盐相关基因的抑制。SES1编码一个未鉴定的内质网(ER)定位蛋白。与其亚细胞分布一致的是，SES1表现出未折叠蛋白反应基因的过度激活，并在很大程度上受到严重内质网胁迫的影响。生化证据表明，SES1是一种重要的分子伴侣，可以缓解盐诱导的内质网胁迫。此外，内质网应激传感器碱性亮氨酸拉链因子17通过直接与其启动子区域结合反式激活SES1。这些结果提供了对盐胁迫反应和内质网动态平衡的见解，并阐明了SES1调节盐抗性的机制。
Salt stress seriously affects plant growth and development. Through genetic screening, we identified and characterized an Arabidopsis (Arabidopsis thaliana) sensitive to scan (ses1) mutant. SES1 was ubiquitously expressed and induced by salt treatment. The salt-sensitive phenotype of ses1 was due neither to the overaccumulation of Na. nor to the suppression of salt tolerance -associated genes. SES1 encoded an uncharacterized endoplasmic reticulum (ER)-localized protein. Coinciding with its subcellular distribution, ses1 exhibited overactivation of unfolded protein response genes and was largely influenced by severe ER stress. Biochemical evidence revealed that SES1 functions as an important molecular chaperone to alleviate salt-induced ER stress. Furthermore, the ER stress sensor basic leucine zipper factorl7 transactivated SES1 by binding directly to its promoter region. These results provide insights into salt stress responses and ER homeostasis and shed light on the mechanism by which SES1 modulates salt resistance.