Two regulatory elements for immunoglobulin kappa light chain gene expression.
Two regulatory elements for immunoglobulin kappa light chain gene expression.
复制标题
免疫球蛋白κ轻链基因表达的两个调控元件。
DOI：
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发表时间：
1984
影响因子：
11.1
通讯作者：
D. Baltimore
中科院分区：
文献类型：
--
作者：
Y. Bergman;D. Rice;R. Grosschedl;D. Baltimore
By using internal deletions within a rearranged immunoglobulin kappa light chain gene, the presence of an intron regulatory sequence (enhancer) has been confirmed. Its presence is required for high-level transcription from a plasmid after transfection into myeloma cells. Transfection efficiency was monitored by the activity of a deleted H4 histone gene included in the plasmid. The intron element could be moved upstream of the gene in both orientations, fulfilling the definition of an enhancer. By using 5' deletions, a second regulatory element was located upstream of the "TATA" box, between positions -69 and -104. These two elements both are required for efficient kappa chain gene expression.
