Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis
Rapid biochemical synthesis of 11C-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis
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DOI：
10.1016/j.bmc.2012.09.038
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发表时间：
2012-11-15
影响因子：
3.5
通讯作者：
Kigawa, Takanori
中科院分区：
文献类型：
--
作者：
Matsuda, Takayoshi;Furumoto, Shozo;Kigawa, Takanori
通过利用抗体的高特异性，免疫PET是用于改进癌症诊断的有前途的方法。在这里，我们提出了一种新的无细胞蛋白质合成方法，用于制备正电子发射体标记的抗体。通过直接掺入临床PET示踪剂L-[C-11]甲硫氨酸，在5分钟内成功地用碳-11（半衰期= 20.4分钟）标记了功能性抗人EGFRvIII单链Fv，MR 1 -1（36%产率）。通过亲和纯化，产物[C-1 - 1] MR 1 -1以高放射化学纯度（>95%）容易且快速地从反应溶液中分离。该方法将广泛适用于制备用于PET成像的放射性标记抗体。(C)2012爱思唯尔有限公司保留所有权利。
Immuno-PET is a promising approach for improved cancer diagnosis, by taking advantage of the high specificity of antibodies. Here, we present a novel cell-free protein synthesis method for preparing a positron emitter labeled-antibody. Functional anti-human EGFRvIII single chain Fv, MR1-1, was successfully labeled with carbon-11 (half-life = 20.4 min) in 5 min (36% yield) by the direct incorporation of the clinical PET tracer, L-[C-11]methionine. The product [C-11]MR1-1 was easily and rapidly isolated with high radiochemical purity (>95%) from the reaction solution, by affinity purification. This method would be widely applicable to the preparation of radiolabeled antibodies for PET imaging. (C) 2012 Elsevier Ltd. All rights reserved.