Effects of trace elements on albumin and lipoprotein glycation in diabetic retinopathy.
Effects of trace elements on albumin and lipoprotein glycation in diabetic retinopathy.
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作者：
N. Hasan
目的 检测某些微量元素对蛋白质和脂蛋白糖基化的影响及其对糖尿病视网膜病变严重程度的影响。方法 2008 年 2 月至 12 月期间，对伊拉克巴格达 Ebin Al-Haitham 眼科疾病专科医院的 42 名糖尿病患者（14 名无视网膜病变 [DC]；14 名患有非增殖性糖尿病视网膜病变 [NPDR]；14 名患有增殖性糖尿病视网膜病变 [PDR]）进行了病例对照研究。此外还有 20 名年龄和性别匹配的健康对照 (NC)。通过琼脂糖凝胶电泳测量白蛋白、α-、前β-和β-脂蛋白的糖化。采用无焰原子吸收分光光度计分析血清中镉（Cd）、硒（Se）、铬（Cr）、锌（Zn）和铜（Cu）的含量。结果 在 PDR 和 NPDR 组中，DC 中的平均血清糖化 β-脂蛋白显着升高（p<0.05），并且糖化白蛋白和前 β-脂蛋白的平均值几乎显着增加（p=0.06）。此外，与 DC 相比，所有糖尿病视网膜病变患者的血清 Cd 平均值 (p<0.05) 和 Zn/Cu 比率 (p<0.001) 均显着降低。 Cd水平随着糖尿病病程(p<0.001)和高血糖(p<0.025)的增加而升高，而血清Cr值随着糖尿病的进展而降低(p<0.025)。结论糖化和氧化过程均参与糖尿病视网膜病变的发生发展，Cd、Se、Cr、Zn、Cu浓度的变化对病情进展有一定影响。
OBJECTIVE To test the effect of some trace elements, on protein and lipoprotein glycosylation and their impact on the severity of diabetic retinopathy. METHODS A case control study was conducted in 42 diabetic patients (14 without retinopathy [DC]; 14 with non-proliferative diabetic retinopathy [NPDR]; 14 with proliferative diabetic retinopathy [PDR]) at Ebin Al-Haitham Specialized Hospital, Baghdad, Iraq for Ocular Diseases from February to December 2008. In addition to 20 age and gender matched healthy controls (NC). The glycation of albumin, alpha-, pre beta-, and beta-lipoproteins was measured by agarose gel electrophoresis. Serum levels of cadmium (Cd), selenium (Se), chromium (Cr), zinc (Zn), and copper (Cu) were analyzed by flameless atomic absorption spectrophotometer. RESULTS There was significant elevation in the mean serum glycated beta-lipoprotein in DC (p<0.05) and a near significant increase (p=0.06) in the means of both glycated albumin and pre beta-lipoproteins among the PDR and NPDR groups. Moreover, a significant reductions in serum means of Cd (p<0.05) and Zn/Cu ratios (p<0.001) were recorded in all diabetic retinopaths as compared to DC. The Cd level rises with the increase in duration of diabetes (p<0.001) and hyperglycemia (p<0.025) whereas, the serum Cr values decreases with the progression of diabetes (p<0.025). CONCLUSION Both glycation and oxidative processes are involved in the development of diabetic retinopathy, and changes in the concentration of Cd, Se, Cr, Zn, and Cu have some impact on the disease progression.