NATURAL HOSTS OF XYLELLA-FASTIDIOSA IN FLORIDA
NATURAL HOSTS OF XYLELLA-FASTIDIOSA IN FLORIDA
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DOI：
10.1094/pd-72-0429
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发表时间：
1988-05-01
期刊：
影响因子：
4.5
通讯作者：
ADLERZ, WC
中科院分区：
文献类型：
--
作者：
HOPKINS, DL;ADLERZ, WC
荧光显微镜，酶联免疫吸附测定，和直接培养，以确定自然感染各种杂草和野生植物与Xylella fastidosa，一种木质部限制的细菌。菌株X.从美国接骨木（Sambucus canadensis）、五叶地锦（Parthenocissus quinquefolia）、胡椒藤（Ampelopsis arborea）、美洲紫珠（Calligonpa americana）和黑莓（Rubus sp.）将其在最初为PD菌株细菌开发的PD3培养基上培养。在PW培养基上，X.对葡萄藤无致病性且不能在PD 3培养基上生长的苛养木从东方巴卡里斯（Baccharis halimifolia）、漆树（Rhus sp.）一枝黄花（Solidago fistulosa）、南方红栎（Quercus falcata）、月桂栎（Q. laurifolia）、水栎（Q. nigra）、桃（Prunus persica）和西卡莫尔（Platanus oceanentalis）。X. fastidiosa发生在佛罗里达，并且需要研究细菌菌株之间的病理学和分类学关系。
Fluorescence microscopy, enzyme-linked immunosorbent assay, and direct culturing were used to determine natural infection of various weeds and wild plants with Xylella fastidosa, a xylem-limited bacterium. Strains of X. fastidiosa that could produce Pierce''s disease symptoms in inoculated grapevine were cultured from American elder (Sambucus canadensis), Virginia creeper (Parthenocissus quinquefolia), peppervine (Ampelopsis arborea), American beautyberry (Callicarpa americana), and blackberry (Rubus sp.). These were cultured on PD3 medium, originally developed for the PD strain bacterium. Using PW medium, strains of X. fastidiosa that were not pathogenic to grapevine and would not grow on PD3 medium were cultured from eastern baccharis (Baccharis halimifolia), sumac (Rhus sp.), goldenrod (Solidago fistulosa), southern red oak (Quercus falcata), laurel oak (Q. laurifolia), water oak (Q. nigra), peach (Prunus persica), and sycamore (Platanus occidentalis). A large number of natural hosts of X. fastidiosa occur in Florida, and there is a need to study pathological and taxonomic relationships among the strains of the bacterium.