Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB.
Structural and Functional Insights into Bacillus subtilis Sigma Factor Inhibitor, CsfB.
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DOI：
10.1016/j.str.2018.02.007
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发表时间：
2018-04-03
期刊：
影响因子：
--
通讯作者：
Isaacson RL
中科院分区：
文献类型：
--
作者：
Martínez-Lumbreras S;Alfano C;Evans NJ;Collins KM;Flanagan KA;Atkinson RA;Krysztofinska EM;Vydyanath A;Jackter J;Fixon-Owoo S;Camp AH;Isaacson RL
细菌基因表达的全局变化可以通过RNA聚合酶的交替σ（σ）因子亚基的协调激活/失活来协调。西格玛因素本身以多种方式进行调节，包括通过反西格玛因素。在这里，我们已经确定了抗σ因子CsfB的溶液结构，该抗σ因子CsfB负责抑制枯草芽孢杆菌孢子形成过程中的两个备选σ因子σG和σE。CsfB组装成对称的同源二聚体，每个单体通过三重-clef锌指折叠与单个Zn 2+离子结合。定向诱变表明，二聚体的形成是至关重要的CsfB介导的抑制σG和σE，我们已经在体外这些相互作用的特点。这项工作代表了我们对CsfB如何介导抑制两种替代σ因子以驱动细菌中发育基因表达的理解的进步。CsfB的结构在抗σ因子中是独特的，CsfB组装成三裂锌指结构域的紧密同源二聚体，CsfB二聚化对于抑制两种替代σ因子是必不可少的。已经解决了抗σ因子CsfB的结构，并探索了其在枯草芽孢杆菌孢子形成过程中抑制两种替代σ因子的作用。这些结果提供了深入了解细菌中基因表达开关的分子机制。
Global changes in bacterial gene expression can be orchestrated by the coordinated activation/deactivation of alternative sigma (σ) factor subunits of RNA polymerase. Sigma factors themselves are regulated in myriad ways, including via anti-sigma factors. Here, we have determined the solution structure of anti-sigma factor CsfB, responsible for inhibition of two alternative sigma factors, σG and σE, during spore formation by Bacillus subtilis. CsfB assembles into a symmetrical homodimer, with each monomer bound to a single Zn2+ ion via a treble-clef zinc finger fold. Directed mutagenesis indicates that dimer formation is critical for CsfB-mediated inhibition of both σG and σE, and we have characterized these interactions in vitro. This work represents an advance in our understanding of how CsfB mediates inhibition of two alternative sigma factors to drive developmental gene expression in a bacterium. The structure of CsfB is unique among anti-sigma factors CsfB assembles into a tight homodimer of treble-clef zinc finger domains CsfB dimerization is essential for inhibition of two alternative sigma factors Martínez-Lumbreras, Alfano et al. have solved the structure of the anti-sigma factor CsfB and explored its role in inhibiting two alternative sigma factors during Bacillus subtilis spore formation. The results provide insight into the molecular mechanism underlying a gene expression switch in bacteria.
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影响因子：
14.9
作者：
Gibson DG
通讯作者：
Gibson DG
DOI：
10.1016/j.bbamcr.2011.06.007
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Baker TA;Sauer RT
通讯作者：
Sauer RT
影响因子：
3.5
作者：
GueroutFleury, AM;Frandsen, N;Stragier, P
通讯作者：
Stragier, P
影响因子：
14.9
作者：
ASLANIDIS, C;DEJONG, PJ
通讯作者：
DEJONG, PJ
影响因子：
14.9
作者：
Grishin, NV
通讯作者：
Grishin, NV