Thiomicrorhabdus heinhorstiae sp. nov. and Thiomicrorhabdus cannonii sp. nov.: novel sulphur-oxidizing chemolithoautotrophs isolated from the chemocline of Hospital Hole, an anchialine sinkhole in Spring Hill, Florida, USA
Thiomicrorhabdus heinhorstiae sp. nov. and Thiomicrorhabdus cannonii sp. nov.: novel sulphur-oxidizing chemolithoautotrophs isolated from the chemocline of Hospital Hole, an anchialine sinkhole in Spring Hill, Florida, USA
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DOI：
10.1099/ijsem.0.005233
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发表时间：
2022-01-01
影响因子：
2.8
通讯作者：
Scott, Kathleen
中科院分区：
文献类型：
--
作者：
Updegraff, Tatum;Schiff-Clark, Grayson;Scott, Kathleen
两个硫氧化，趋化岩石自养需氧菌从位于佛罗里达州Weeki Wachee河内的一个anchiine天坑的趋化cline中分离出来。两株革兰氏染色阴性细胞均为可运动的趋化棒。16S rRNA基因的系统发育分析、核糖体蛋白的预测氨基酸序列、平均核苷酸身份和比对分数表明，菌株HH1(T)和HH3(T)代表属于硫微habdus属的新物种。HH1(T)的G+C片段为47.8 mol%，基因组长度为2.61 Mb; HH3(T)的G+C片段为52.4 mol%，基因组长度为2.49 Mb。两菌株的主要脂肪酸为C-16（∶1）、C-18:1和C-16(∶0)，在HH1(T)和HH3(T)中分别添加了C-10:0 3-OH和C-12（∶0）。这两种菌株的趋化石自养生长均由单质硫、硫化物、四硫酸盐和硫代硫酸盐支持，并且h1 (T)也能够利用分子氢。这两种菌株都不能异养生长或使用硝酸盐作为终端电子受体。菌株HH1(T)从pH 6.5生长到8.5，最适pH为7.4，而菌株HH3(T)从pH 6生长到8，最适pH为7.5。HH1(T)和HH3(T)的最佳生长温度分别为32.8°C和32°C。HH1(T)在（NaCl） 80 ~ 689 mM的培养基中生长，最适为400mM， HH3(T)在80 ~ 517mm的培养基中生长，最适为80 mM。提出了HH1(T) （=DSM 111584（T)=ATCC TSD-240(T)）型菌株命名为Thiomicrorhabdus heinhorstiae sp. nov.）。建议将其命名为硫微habdus cannonii sp. nov，类型菌株为HH3(T) (=DSM 111593（T)=ATCC TSD-241(T)）。
Two sulphur-oxidizing, chemolithoautotrophic aerobes were isolated from the chemocline of an anchialine sinkhole located within the Weeki Wachee River of Florida. Gram-stain-negative cells of both strains were motile, chemotactic rods. Phylogenetic analysis of the 16S rRNA gene and predicted amino acid sequences of ribosomal proteins, average nucleotide identities, and alignment fractions suggest the strains HH1(T) and HH3(T) represent novel species belonging to the genus Thiomicrorhabdus. The genome G+C fraction of HH1(T) is 47.8 mol% with a genome length of 2.61 Mb, whereas HH3(T) has a G+C fraction of 52.4 mol% and 2.49 Mb genome length. Major fatty acids of the two strains included C-16(:1), C-18:1, and C-16(:0), with the addition of C-10:0 3-OH in HH1(T) and C-12(:0) in HH3(T). Chemolithoautotrophic growth of both strains was supported by elemental sulphur, sulphide, tetrathionate, and thiosulphate, and HH1(T) was also able to use molecular hydrogen. Neither strain was capable of heterotrophic growth or use of nitrate as a terminal electron acceptor. Strain HH1(T) grew from pH 6.5 to 8.5, with an optimum of pH 7.4, whereas strain HH3(T) grew from pH 6 to 8 with an optimum of pH 7.5. Growth was observed between 15-35 degrees C with optima of 32.8 degrees C for HH1(T) and 32 degrees C for HH3(T). HH1(T) grew in media with (NaCl) 80-689 mM, with an optimum of 400mM, while HH3(T) grew at 80-517mM, with an optimum of 80 mM. The name Thiomicrorhabdus heinhorstiae sp. nov. is proposed, and the type strain is HH1(T) (=DSM 111584(T)=ATCC TSD-240(T)). The name Thiomicrorhabdus cannonii sp. nov is proposed, and the type strain is HH3(T) (=DSM 111593(T)=ATCC TSD-241(T)).