Glycopeptides from brain inhibit rates of polypeptide chain elongation.
Glycopeptides from brain inhibit rates of polypeptide chain elongation.
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来自大脑的糖肽抑制多肽链延长的速率。
DOI：
10.1016/s0021-9258(18)43746-5
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
T. Johnson
中科院分区：
文献类型：
--
作者：
R. Kinders;J. Hughes;T. Johnson
在以前的报告中，我们已经从小鼠大脑中鉴定出细胞表面糖肽(BCSG)，它可以抑制几种细胞类型的蛋白质合成和有丝分裂。当乳鼠肾(BHK)-21细胞感染水泡性口炎病毒(一种负链RNA病毒)时，BCSG广泛地抑制病毒蛋白的合成。这种抑制对蛋白质和糖蛋白的合成都是有效的，并且与感染细胞对氨基酸的摄取、病毒RNA的合成和病毒蛋白的降解无关。对未感染的BHK-21细胞的多聚核糖体图谱分析表明，细胞蛋白质合成的抑制程度不能归因于核糖核酸酶的激活或仅归因于链起始的中断。当直接加入来自BHK-21细胞的无细胞蛋白质合成系统时，BCSG是无效的，但如果首先允许抑制物质与细胞反应，则无细胞蛋白质合成显著减少。当BCSG与细胞在0℃下反应5min时，受试细胞BHK-21(BCSG敏感株)和小鼠纤维肉瘤2237(BCSG不敏感株)均有效地吸附了培养液中的抑制物。
In previous reports, we have identified cell-surface glycopeptides from mouse cerebrum (BCSG) that inhibited protein synthesis and mitosis in several cell types. When baby hamster kidney (BHK)-21 cells were infected with vesicular stomatitis virus (a negative strand RNA virus), BCSG extensively inhibited viral protein synthesis. This inhibition was effective against both protein and glycoprotein synthesis and was independent of amino acid uptake by infected cells, synthesis of viral RNA, and degradation of viral proteins. Analysis of polyribosome profiles in uninfected BHK-21 cells indicated that the degree of cellular protein synthesis inhibition could not be attributed to activation of RNase or solely to a disruption of chain initiation. When added directly to a cell-free protein-synthesizing system derived from BHK-21 cells, BCSG was ineffective, but if the inhibitory material was first allowed to react with cells, cell-free protein synthesis was substantially reduced. When BCSG were reacted with cells for 5 min at 0 degrees C, the cells tested, BHK-21 (a BCSG-sensitive line) and murine fibrosarcoma 2237 (a BCSG-insensitive line), both effectively adsorbed the inhibitor from the medium.