Proteome Alterations of Hippocampal Cells Caused by Clostridium botulinum C3 Exoenzyme.
Proteome Alterations of Hippocampal Cells Caused by Clostridium botulinum C3 Exoenzyme.
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肉毒梭菌 C3 胞外酶引起的海马细胞蛋白质组改变
DOI：
10.1021/acs.jproteome.5b00591
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发表时间：
2015
影响因子：
4.4
通讯作者：
Pich A
中科院分区：
文献类型：
--
作者：
Schröder;Rohrbeck A;Just I;Pich A
肉毒梭菌C3 bot是一种细菌单ADP核糖基化酶，它将ADP核糖部分转移到小GTP酶Rho A/B/C上。C3 bot和催化失活突变体（C3 E174 Q）引起原代海马神经元中的轴突和树突生长以及分支。在培养的鼠海马HT 22细胞中，使用鸟枪蛋白质组学方法分析响应于C3 bot或C3 E174 Q处理的蛋白质丰度。在6天内的4个时间点进行蛋白质组分析。在每个时间点鉴定超过4000个蛋白质组，并在一式三份分析中定量。在第一天，46种蛋白质显示出丰度的改变，6天后，超过700种蛋白质对C3 bot产生了上调或下调的反应。相反，C3 E174 Q对蛋白质丰度没有可证实的影响。通过多反应监测验证几种蛋白质的蛋白质定量。对改变的蛋白质的数据分析揭示了受C3 bot影响的不同细胞过程。它们特别参与线粒体和溶酶体过程、粘附、碳水化合物和葡萄糖代谢、信号转导以及翻译和核糖体生物合成的核蛋白。本研究结果为C3 bot在海马细胞中的功能提供了新的见解。
C3bot fromClostridium botulinumis a bacterial mono-ADP-ribosylating enzyme, which transfers an ADP-ribose moiety onto the small GTPases Rho A/B/C. C3bot and the catalytic inactive mutant (C3E174Q) cause axonal and dendritic growth as well as branching in primary hippocampal neurons. In cultured murine hippocampal HT22 cells, protein abundances were analyzed in response to C3bot or C3E174Q treatment using a shotgun proteomics approach. Proteome analyses were performed at four time points over 6 days. More than 4000 protein groups were identified at each time point and quantified in triplicate analyses. On day one, 46 proteins showed an altered abundance, and after 6 days, more than 700 proteins responded to C3bot with an up- or down-regulation. In contrast, C3E174Q had no provable impact on protein abundance. Protein quantification was verified for several proteins by multiple reaction monitoring. Data analysis of altered proteins revealed different cellular processes that were affected by C3bot. They are particularly involved in mitochondrial and lysosomal processes, adhesion, carbohydrate and glucose metabolism, signal transduction, and nuclear proteins of translation and ribosome biogenesis. The results of this study gain novel insights into the function of C3bot in hippocampal cells.
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影响因子：
2
作者：
Jochim, Nelli;Gerhard, Ralf;Pich, Andreas
通讯作者：
Pich, Andreas
影响因子：
3.7
作者：
Rohrbeck A;Schröder A;Hagemann S;Pich A;Höltje M;Ahnert-Hilger G;Just I
通讯作者：
Just I
来自金黄色葡萄球菌的新型 C3 样 ADP-核糖基转移酶修饰 RhoE 和 Rnd3*
影响因子：
4.8
作者：
C. Wilde;G. S. Chhatwal;G. Schmalzing;K. Aktories;I. Just
通讯作者：
I. Just
影响因子：
4.4
作者：
J. Zeiser;R. Gerhard;I. Just;A. Pich
通讯作者：
J. Zeiser;R. Gerhard;I. Just;A. Pich
DOI：
10.1016/j.jchromb.2008.12.005
发表时间：
2009-05-01
影响因子：
3
作者：
Muetzelburg, M. V.;Hofmann, F.;Pich, A.
通讯作者：
Pich, A.