Transcription sites are not correlated with chromosome territories in wheat nuclei.
Transcription sites are not correlated with chromosome territories in wheat nuclei.
复制标题
转录位点与小麦核中的染色体领土无关。
DOI：
10.1083/jcb.143.1.5
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发表时间：
1998-10-05
影响因子：
7.8
通讯作者：
Shaw, P J
中科院分区：
文献类型：
--
作者：
Abranches, R;Beven, A F;Aragon-Alcaide, L;Shaw, P J
我们已经确定了整体核结构，染色体领土，核内的转录位点之间的关系，利用三维共聚焦显微镜保存完好的小麦根组织切片。以黑麦基因组探针为探针，利用GISH技术对小麦/黑麦易位系和附加系的染色体区域进行了可视化研究。染色体表现为细长的区域，并显示出明显的着丝粒-端粒极化，两条可视化的染色体在细胞核中彼此大致平行。用探针标记端粒和着丝粒证实了所有细胞中引人注目的Rabl构型，具有清晰的着丝粒聚类，并且细胞文件通常在几个分裂周期中保持共同的极性。通过BrUTP掺入未固定的组织切片中检测转录位点，并揭示了均匀分布在整个核质中的许多病灶的模式，以及在核仁中更强烈标记的病灶。小麦染色体上的基因富集区可能集中在端粒附近。然而，我们没有发现任何迹象表明端粒和着丝粒极的细胞核之间的转录位点的浓度的差异。我们也不能检测到任何证据表明，转录位点优先本地化的染色体领土边界。
We have determined the relationship between overall nuclear architecture, chromosome territories, and transcription sites within the nucleus, using three-dimensional confocal microscopy of well preserved tissue sections of wheat roots. Chromosome territories were visualized by GISH using rye genomic probe in wheat/rye translocation and addition lines. The chromosomes appeared as elongated regions and showed a clear centromere–telomere polarization, with the two visualized chromosomes lying approximately parallel to one another across the nucleus. Labeling with probes to telomeres and centromeres confirmed a striking Rabl configuration in all cells, with a clear clustering of the centromeres, and cell files often maintained a common polarity through several division cycles. Transcription sites were detected by BrUTP incorporation in unfixed tissue sections and revealed a pattern of numerous foci uniformly distributed throughout the nucleoplasm, as well as more intensely labeled foci in the nucleoli. It has been suggested that the gene-rich regions in wheat chromosomes are clustered towards the telomeres. However, we found no indication of a difference in concentration of transcription sites between telomere and centromere poles of the nucleus. Neither could we detect any evidence that the transcription sites were preferentially localized with respect to the chromosome territorial boundaries.