Revealing the nanoindentation response of a single cell using a 3D structural finite element model
Revealing the nanoindentation response of a single cell using a 3D structural finite element model
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DOI：
10.1557/s43578-020-00004-5
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发表时间：
2021-01
影响因子：
2.7
通讯作者：
Wenjian Yang;D. Lacroix;L. P. Tan;Jinju Chen
中科院分区：
文献类型：
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作者：
Wenjian Yang;D. Lacroix;L. P. Tan;Jinju Chen
据报道，细胞表观模量的变化与细胞异常和疾病相关。压痕通常用于测量这些模量;然而，有证据表明，所采用的压痕协议会影响测量的模量，这会影响我们对生理条件如何调节细胞力学的理解。大多数研究将细胞视为均质材料或由细胞质和细胞核组成的简单核壳结构：两者都远离细胞的真实的结构。为了研究压痕协议依赖的细胞力学，关键的细胞内组件（皮质层，细胞质，肌动蛋白应力纤维，微管，和细胞核）的有限元模型，而不是已经开发。结果表明，锥形压头获得的表观模量随着锥角的增加而降低，但是，这种变化不太明显的球形压头的半径增加。此外，还研究了压头几何形状与细胞内组分之间的相互作用，这有助于理解细胞的结构-力学-功能关系。
Changes in the apparent moduli of cells have been reported to correlate with cell abnormalities and disease. Indentation is commonly used to measure these moduli; however, there is evidence to suggest that the indentation protocol employed affects the measured moduli, which can affect our understanding of how physiological conditions regulate cell mechanics. Most studies treat the cell as a homogeneous material or a simple core–shell structure consisting of cytoplasm and a nucleus: both are far from the real structure of cells. To study indentation protocol-dependent cell mechanics, a finite element model of key intracellular components (cortex layer, cytoplasm, actin stress fibres, microtubules, and nucleus) has instead been developed. Results have shown that the apparent moduli obtained with conical indenters decreased with increasing cone angle; however, this change was less significant for spherical indenters of increasing radii. Furthermore, the interplay between indenter geometry and intracellular components has also been studied, which is useful for understanding structure-mechanics-function relationships of cells.