A strategy for annotating the human milk glycome
A strategy for annotating the human milk glycome
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DOI：
10.1021/jf0615810
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发表时间：
2006-10-04
影响因子：
6.1
通讯作者：
Lebrilla, Carlito B.
中科院分区：
文献类型：
--
作者：
Ninonuevo, Milady R.;Park, Youmie;Lebrilla, Carlito B.
人乳中的寡聚肽代表了一组生物活性分子，它们已经进化成为人乳中丰富多样的组分，尽管它们对婴儿没有直接的营养价值。最近的一个假说提出，它们可能是肠道微生物菌群和粘膜免疫系统发育的底物。无法确定这些寡糖的确切组成限制了研究和理解其生物功能的能力。通过微芯片液相色谱/质谱（HPLC-Chip/MS）和基质辅助激光解吸/电离傅里叶变换离子回旋共振质谱（MALDI-FT ICR MS）等技术组合，分析了从个体人乳样品的脂质和蛋白质中分离的寡聚肽。使用正交飞行时间（o-TOF）质谱法获得的精确质量测量值提供了约200种单个分子种类的寡糖组成。比较HPLC-Chip/MS图谱从五个不同的妇女揭示了牛奶低聚糖组成的变化。HPLC-Chip/MS分析提供了一种常规鉴定牛奶低聚糖的方法。串联质谱结合外切糖苷酶消化可明确区分结构异构体。
Oligosaccharides in human milk represent a group of bioactive molecules that have evolved to be an abundant and diverse component of human milk, even though they have no direct nutritive value to the infant. A recent hypothesis proposes that they could be substrates for the development of the intestinal microflora and the mucosal immune system. The inability to determine the exact composition of these oligosaccharides limits research and the ability to understand their biological functions. Oligosaccharides isolated from the lipids and proteins of individual human milk samples were analyzed by a combination of techniques including microchip liquid chromatography mass spectrometry (HPLC-Chip/MS) and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FT ICR MS). Accurate mass measurements obtained using an orthogonal time-of-flight (o-TOF) mass spectrometry provided oligosaccharide composition for approximately 200 individual molecular species. Comparison of HPLC-Chip/MS profiles from five different women revealed variations in milk oligosaccharide compositions. HPLC-Chip/MS profiling provides a method for routinely identifying milk oligosaccharides. Tandem MS in combination with exoglycosidase digestion provides unambiguous differentiation of structural isomers.