Effects of 4-META/MMA-TBB Resin at Different Curing Stages on Osteoblasts and Gingival Epithelial Cells
Effects of 4-META/MMA-TBB Resin at Different Curing Stages on Osteoblasts and Gingival Epithelial Cells
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DOI：
10.3290/j.jad.a35839
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发表时间：
2016-01-01
影响因子：
3.3
通讯作者：
Kitamura, Chiaki
中科院分区：
文献类型：
--
作者：
Morotomi, Takahiko;Hirata-Tsuchiya, Shizu;Kitamura, Chiaki
目的：材料与方法：将4-Meta/MMA-TBB树脂与不同固化阶段的Super-Bond C和B混合物共同培养于MC 3 T3-E1小鼠前成骨细胞系和GE-1小鼠牙龈上皮细胞系，检测细胞活力。结果：4-Meta/MMA-TBB树脂处理的MC 3 T3-E1细胞在固化初期（固化1 min），大部分细胞死亡，无ALP活性。4-Meta/MMA-TBB树脂的后期固化阶段（固化7分钟）在第1天显示出细胞毒性，但毒性作用是暂时的，并且细胞中的增殖能力和ALP活性在第7天与对照细胞相似。完全固化的4-Meta/MMA-TBB树脂（固化1或12小时后）不影响MC 3 T3-E1细胞的细胞活力或ALP活性。结论：虽然4-Meta/MMA-TBB树脂在固化初期对MC 3 T3-E1细胞有毒性作用，但在固化后期对细胞的毒性作用很小。相比之下，未固化或固化的树脂都不影响GE-1电池。
Purpose: To assess the effects of different curing stages of 4-META/MMA-TBB resin on osteoblasts and gingival keratinocytes.Materials and Methods: The MC3T3-E1 murine pre-osteoblastic cell line and GE-1 murine gingival epithelial cell line were cultured with mixtures of Super-Bond C&B at different curing stages, and the cell viability was assessed. The alkaline phosphatase (ALP) activity of the MC3T3-E1 cells was also assessed.Results: The majority of the MC3T3-E1 cells died and showed no ALP activity when cultured with 4-META/MMA-TBB resin during the initial curing phase (1 min of curing). A later curing phase of the 4-META/MMA-TBB resin (7 min of curing) showed cytotoxicity at day 1, but the toxic effect was temporary and the proliferative capacity and ALP activity in the cells were similar to control cells at day 7. Completely cured 4-META/MMA-TBB resin (after 1 or 12 h of curing) did not affect the cell viability or ALP activity of the MC3T3-E1 cells. In contrast, 4-META/MMA-TBB resin showed no effect on the GE-1 cells at any stage of curing.Conclusion: Although 4-META/MMA-TBB resin during the initial curing phase shows toxic effects on MC3T3-E1 cells, that cytotoxicity is minimal at later curing phases. In contrast, neither the uncured nor cured resins affected the GE-1 cells.