Chiral Discrimination of Branched-chain Fatty Acids by Reversed-phase HPLC after Labeling with a Chiral Fluorescent Conversion Reagent
Chiral Discrimination of Branched-chain Fatty Acids by Reversed-phase HPLC after Labeling with a Chiral Fluorescent Conversion Reagent
复制标题
使用手性荧光转换试剂标记后，通过反相 HPLC 进行支链脂肪酸的手性鉴别
DOI：
10.1271/bbb.68.153
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
H. Ohrui
中科院分区：
文献类型：
--
作者：
K. Akasaka;H. Ohrui
用手性荧光转化试剂(1R,2R)-和(1S,2S)-2-（2,3-蒽二甲氧基）环己醇标记含有16 ~ 29个碳原子的Anteiso脂肪酸。在低柱温条件下，含有20个碳原子的蚁酸的非对映异构体酯在ODS柱上被分离成两个峰，而含有21个碳原子以上的非对映异构体酯则未被分离。C30色谱柱可以分离到C29 anteisoacid的非对映异构体酯。通过衍生物的洗脱顺序可以预测每种酸的绝对构型。
Anteiso fatty acids having 16 to 29 carbon atoms were labeled with the chiral fluorescent conversion reagents, (1R,2R)- and (1S,2S)-2-(2,3-anthracenedicarboximido)cyclohexanol. The diastereomeric esters of anteiso acids having up to 20 carbon atoms were separated into two peaks in an ODS column under low column-temperature conditions, while those having more than 21 carbon atoms were not separated. A C30 column made it possible to separate diastereomeric esters up to C29 anteiso acid. It was possible to predict the absolute configuration of each acid by the elution order of the derivatives.