Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
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DOI：
10.1242/dev.00973
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发表时间：
2004-02-01
期刊：
影响因子：
4.6
通讯作者：
Nakano, T
中科院分区：
文献类型：
--
作者：
Kuramochi-Miyagawa, S;Kimura, T;Nakano, T
由保守的PAZ和Piwi结构域定义的piwi家族基因在各种生物体中的干细胞自我更新、RNA沉默和翻译调控中起重要作用。为了揭示piwi的哺乳动物同源物的功能，我们产生并分析了在Mili基因中具有靶向突变的小鼠，Mili基因是piwi的三种小鼠同源物之一。MILI基因敲除小鼠的精子发生在第一次减数分裂的早期前期（从偶线期到粗线期早期）被完全阻断，小鼠不育。然而，原始生殖细胞发育和雌性生殖细胞的生产没有受到干扰。此外，MILI结合MVH，这是一个必要的因素，在早期精母细胞阶段。MILI和MVH缺陷小鼠的表型和MILI和MVH的物理结合特性的相似性表明这些蛋白质在转录后调节中的功能关联。这些数据表明，MILI是必要的精母细胞的分化。
The piwi family genes, which are defined by conserved PAZ and Piwi domains, play important roles in stem cell self-renewal, RNA silencing, and translational regulation in various organisms. To reveal the function of the mammalian homolog of piwi, we produced and analyzed mice with targeted mutations in the Mili gene, which is one of three mouse homologs of piwi. Spermatogenesis in the MILI-null mice was blocked completely at the early prophase of the first meiosis, from the zygotene to early pachytene, and the mice were sterile. However, primordial germ cell development and female germ cell production were not disturbed. Furthermore, MILI bound to MVH, which is an essential factor during the early spermatocyte stage. The similarities in the phenotypes of the MILI- and MVH-deficient mice and in the physical binding properties of MILI and MVH indicate a functional association of these proteins in post-transcriptional regulation. These data indicate that MILI is essential for the differentiation of spermatocytes.