Bacillus arsenicoselenatis, sp nov, and Bacillus selenitireducens, sp nov:: two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic
Bacillus arsenicoselenatis, sp nov, and Bacillus selenitireducens, sp nov:: two haloalkaliphiles from Mono Lake, California that respire oxyanions of selenium and arsenic
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DOI：
10.1007/s002030050673
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发表时间：
1998-12-01
影响因子：
2.8
通讯作者：
Oremland, RS
中科院分区：
文献类型：
--
作者：
Blum, JS;Bindi, AB;Oremland, RS
从加州莫诺湖（Mono Lake）的缺氧淤泥中分离到两株革兰氏阳性厌氧细菌（菌株E1 H和MLS 10）。两者都是通过异化还原As（V）为As（III），同时将乳酸盐氧化为乙酸盐和CO2来生长的。砷硒芽孢杆菌（菌株E1 H）是一种孢子形成棒，也通过异化还原Se（VI）为Se（IV）生长。芽孢杆菌（Bacillus selenitire-ducens）（菌株MLS 10）是一种短的、不形成孢子的杆，其通过Se（IV）异化还原为Se（0）而生长。当两个分离物共培养时，实现了Se（VI）完全还原为Se（0）。这两种菌株都是嗜碱菌，在pH 8.5-10范围内具有最佳的比生长速率。菌株E1 H的最适盐度为60 g l（-1）NaCl，而菌株MLS 10的最适生长盐度较低（24-60 g l（-1）NaCl）。这两种菌株与电子供体和受体一起生长的能力有限，而不是上面给出的那些。菌株MLS IO表现出作为微需氧菌的弱生长，并且还能够在葡萄糖上发酵生长，而菌株E1 H是严格的厌氧菌。比较16 S rRNA基因序列分析将这两个分离物与其他芽孢杆菌属物种。在低G+C革兰氏阳性菌群中。
Two gram-positive anaerobic bacteria (strains E1H and MLS10) were isolated from the anoxic muds of Mono Lake, California, an alkaline, hypersaline, arsenic-rich water body. Both grew by dissimilatory reduction of As(V) to As(III) with the concomitant oxidation of lactate to acetate plus CO2. Bacillus arsenicoselenatis (strain E1H) is a spore-forming rod that also grew by dissimilatory reduction of Se(VI) to Se(IV). Bacillus selenitire-ducens (strain MLS10) is a short, non-spore-forming rod that grew by dissimilatory reduction of Se(IV) to Se(0). When the two isolates were cocultured, a complete reduction of Se(VI) to Se(0) was achieved. Both isolates are alkaliphiles and had optimal specific growth rates in the pH range of 8.5-10. Strain E1H had a salinity optimum at 60 g l(-1) NaCl, while strain MLS10 had optimal growth at lower salinities (24-60 g l(-1) NaCl). Both strains have limited abilities to grow with electron donors and accepters other than those given above. Strain MLS IO demonstrated weak growth as a microaerophile and was also capable of fermentative growth on glucose, while strain E1H is a strict anaerobe. Comparative 16S rRNA gene sequence analysis placed the two isolates with other Bacillus spp. in the low G+C gram-positive group of bacteria.