A fungal powdery mildew pathogen induces extensive local and marginal systemic changes in the Arabidopsis thaliana microbiota
A fungal powdery mildew pathogen induces extensive local and marginal systemic changes in the Arabidopsis thaliana microbiota
复制标题
DOI：
10.1111/1462-2920.15768
复制
发表时间：
2021-09-27
影响因子：
5.1
通讯作者：
Panstruga, Ralph
中科院分区：
文献类型：
--
作者：
Duran, Paloma;Reinstaedler, Anja;Panstruga, Ralph
白粉病是由附生营养专性子囊菌引起的一种叶部病害。白粉菌定植如何在当地和系统地影响寄主微生物群落的研究仍然很少。我们对生长在自然土壤和灵知生菌系统中的拟南芥进行了白粉病侵染实验，研究了病原菌入侵对天然多王国或合成细菌群落(Syncoms)的影响。我们发现，在土生植物侵染后，龙须草的竞争能力超过了许多与植物共生的真菌，而根部真菌群落结构保持不变。我们进一步检测到在这种设置中叶相关细菌群落的显着变化，但没有检测到与根相关的细菌群落的显著变化。用细菌叶衍生的Syncom预定殖无菌海桑叶片，然后侵染G.orontii，引起了叶片细菌微生物群的总体上类似的转变和与根相关的细菌组合的微小变化。然而，根样本中的直立根来源的Syncom对叶片感染G.orontii仍然保持着强大的抵抗力。虽然病原菌的生长不受叶片合并症的影响，但真菌侵染导致了叶片细菌负荷的两倍增加。我们的发现表明，G orontii感染主要影响当地植物组织中的微生物群落，可能是由病原体诱导的源-库关系和宿主免疫状态的变化所驱动的。
Powdery mildew is a foliar disease caused by epiphytically growing obligate biotrophic ascomycete fungi. How powdery mildew colonization affects host resident microbial communities locally and systemically remains poorly explored. We performed powdery mildew (Golovinomyces orontii) infection experiments with Arabidopsis thaliana grown in either natural soil or a gnotobiotic system and studied the influence of pathogen invasion into standing natural multi-kingdom or synthetic bacterial communities (SynComs). We found that after infection of soil-grown plants, G. orontii outcompeted numerous resident leaf-associated fungi while fungal community structure in roots remained unaltered. We further detected a significant shift in foliar but not root-associated bacterial communities in this setup. Pre-colonization of germ-free A. thaliana leaves with a bacterial leaf-derived SynCom, followed by G. orontii invasion, induced an overall similar shift in the foliar bacterial microbiota and minor changes in the root-associated bacterial assemblage. However, a standing root-derived SynCom in root samples remained robust against foliar infection with G. orontii. Although pathogen growth was unaffected by the leaf SynCom, fungal infection caused a twofold increase in leaf bacterial load. Our findings indicate that G. orontii infection affects mainly microbial communities in local plant tissue, possibly driven by pathogen-induced changes in source-sink relationships and host immune status.