Enhanced nitric oxide and reactive oxygen species production and damage after inhalation of silica.
Enhanced nitric oxide and reactive oxygen species production and damage after inhalation of silica.
复制标题
吸入二氧化硅后增强一氧化氮和活性氧的产生和损害。
DOI：
10.1152/ajplung.00427.2001
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
V. Castranova
中科院分区：
文献类型：
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作者：
D. Porter;L. Millecchia;V. Robinson;A. Hubbs;P. Willard;D. Pack;D. Ramsey;J. McLaurin;A. Khan;D. Landsittel;A. Teass;V. Castranova
在该研究之前的报告中，详细介绍了肺部炎症、支气管肺泡灌洗细胞细胞因子产生和核因子κ B激活、细胞毒性损伤和纤维化的测量。在这项研究中，我们研究了大鼠吸入二氧化硅、一氧化氮（NO）和活性氧（ROS）产生与这些自由基介导的损伤之间的时间关系。大鼠暴露于二氧化硅气溶胶（15 mg/m(3)二氧化硅，6小时/天，5天/周），持续116天。我们报道了以下方面的时间依赖性变化：1)肺泡巨噬细胞的激活和伴随的NO和ROS的产生，2)诱导的NO合成酶和NO诱导的损伤产物硝基酪氨酸的免疫组织化学定位，3)支气管肺泡灌洗液NO(x)和超氧化物歧化酶浓度，4)肺脂质过氧化水平。本研究的主要观察结果如下：1)二氧化硅暴露时NO和ROS的产生和由此引起的损伤增加；2)诱导NO合成酶激活位点和NO介导的损伤与肺部病理病变在解剖学上相关。
In previous reports from this study, measurements of pulmonary inflammation, bronchoalveolar lavage cell cytokine production and nuclear factor-kappa B activation, cytotoxic damage, and fibrosis were detailed. In this study, we investigated the temporal relationship between silica inhalation, nitric oxide (NO), and reactive oxygen species (ROS) production, and damage mediated by these radicals in the rat. Rats were exposed to a silica aerosol (15 mg/m(3) silica, 6 h/day, 5 days/wk) for 116 days. We report time-dependent changes in 1) activation of alveolar macrophages and concomitant production of NO and ROS, 2) immunohistochemical localization of inducible NO synthase and the NO-induced damage product nitrotyrosine, 3) bronchoalveolar lavage fluid NO(x) and superoxide dismutase concentrations, and 4) lung lipid peroxidation levels. The major observations made in this study are as follows: 1) NO and ROS production and resultant damage increased during silica exposure, and 2) the sites of inducible NO synthase activation and NO-mediated damage are associated anatomically with pathological lesions in the lungs.
影响因子：
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作者：
M. Grisham;G. Johnson;J. R. Lancaster
通讯作者：
M. Grisham;G. Johnson;J. R. Lancaster