Hypothalamic hypocretin/orexin and neuropeptide Y: Divergent interaction with energy depletion and leptin
Hypothalamic hypocretin/orexin and neuropeptide Y: Divergent interaction with energy depletion and leptin
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DOI：
10.1006/bbrc.1999.0605
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发表时间：
1999-04-29
影响因子：
3.1
通讯作者：
Richy, S
中科院分区：
文献类型：
--
作者：
Beck, B;Richy, S
本研究的目的是测量长期瘦素治疗对下丘脑中存在的两种促食欲肽（即下丘脑分泌素/食欲素和神经肽 Y (NPY)）的影响。为此，将重组鼠瘦素（0.2 毫克/大鼠/天）或盐水连续 7 天向 Long-Evans 大鼠腹膜内注射。瘦素治疗的大鼠的食物摄入量（-8%；p < 0.002）和体重增加（23.7 +/- 1 vs 31.5 +/- 1.3 g；p < 0.003）显着低于盐水治疗的大鼠。在任何显微解剖的脑区域（包括弓状核和室旁核）中，NPY 浓度没有显着变化。瘦素治疗显着降低了外侧下丘脑的食欲素 A 浓度（-68%；p < 0.01）。在与瘦素处理的大鼠配对喂养的盐水处理的大鼠中，也观察到较小的下降（-46%；p < 0.04）。我们的结论是，食欲素/下丘脑分泌素可被视为中枢神经系统中瘦素的新中继。在配对大鼠中观察到的能量供应较低的情况下的变化可能构成大脑的警报系统，因此被认为是建立针对能量消耗的防御机制的第一步。 (C) 1999 年学术出版社。
The aim of this study was to measure the effects of chronic leptin treatment on two orexigenic peptides present in the hypothalamus namely hypocretin/orexin and neuropeptide Y (NPY), For this purpose, recombinant murine leptin (0.2 mg/rat/day) or saline were injected intraperitoneally in Long-Evans rats for 7 consecutive days. Food intake (-8%; p < 0.002) and body weight gain (23.7 +/- 1 vs 31.5 +/- 1.3 g; p < 0.003) were significantly lower in leptin-treated rats than the saline-treated rats. NPY concentrations did not change significantly in any of the microdissected brain areas including the arcuate and paraventricular nuclei. Orexin A concentration in the lateral hypothalamus was significantly decreased by the leptin treatment (-68%; p < 0.01). A smaller decrease (-46%; p < 0.04) was also noted in saline-treated rats pairfed to the level of the leptin-treated rats. We conclude that orexin/hypocretin could be considered as a new relay for leptin in the central nervous system. Its variation in case of lower energy supply observed in pairfed rats could constitute an alerting system for the brain and therefore considered as the first step in the establishment of defense mechanisms against energy depletion. (C) 1999 Academic Press.