The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development
The Molecular Basis of Radial Intercalation during Tissue Spreading in Early Development
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DOI：
10.1016/j.devcel.2016.04.008
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发表时间：
2016-05-09
影响因子：
11.8
通讯作者：
Mayor, Roberto
中科院分区：
文献类型：
--
作者：
Szabo, Andras;Cobo, Isidoro;Mayor, Roberto
径向嵌入是一个基本的过程，负责在大规模形态发生的多层组织变薄，然而，其分子机制仍然是难以捉摸的。使用两栖动物epiboly，在原肠胚形成过程中的动物半球的变薄和扩展，在这里，我们提供的证据表明，径向嵌入是由细胞向组织的外层趋化性驱动的。趋化性在组织伸展和变薄中的作用不同于其与细胞的大距离定向运动相关的典型作用。我们确定的化学引诱物作为补体成分C3a，一个因素通常与免疫系统。该机制是通过计算建模和测试，在体内，离体和体外。这种机制对化学引诱物水平和表达模式的波动是鲁棒的，并解释了外延期间的扩张。这项研究提供了深入了解径向嵌入的基本过程，并可应用于广泛的形态发生事件。
Radial intercalation is a fundamental process responsible for the thinning of multilayered tissues during large-scale morphogenesis; however, its molecular mechanism has remained elusive. Using amphibian epiboly, the thinning and spreading of the animal hemisphere during gastrulation, here we provide evidence that radial intercalation is driven by chemotaxis of cells toward the external layer of the tissue. This role of chemotaxis in tissue spreading and thinning is unlike its typical role associated with large-distance directional movement of cells. We identify the chemoattractant as the complement component C3a, a factor normally linked with the immune system. The mechanism is explored by computational modeling and tested in vivo, ex vivo, and in vitro. This mechanism is robust against fluctuations of chemoattractant levels and expression patterns and explains expansion during epiboly. This study provides insight into the fundamental process of radial intercalation and could be applied to a wide range of morphogenetic events.