Evidence for the ability of hippocampal neurons to develop acute tolerance to ethanol in behaving rats.
Evidence for the ability of hippocampal neurons to develop acute tolerance to ethanol in behaving rats.
复制标题
行为大鼠海马神经元对乙醇产生急性耐受能力的证据。
DOI：
10.1016/s0006-8993(01)02319-8
复制
发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Bungay,PM
中科院分区：
文献类型：
--
作者：
Ludvig,N;George,MA;Tang,HM;Gonzales,RA;Bungay,PM
BackgroundThe cellular mechanisms underlying acute tolerance to alcohol are unclear. This study aimed to determine whether hippocampal neurons have the ability to develop acute tolerance to alcohol in behaving rats.MethodsIntrahippocampal microdialysis was performed in freely behaving rats, and the firing of single neurons in the dialysis area was recorded. The control microdialysis fluid, artificial cerebrospinal fluid (ACSF), was replaced with 1 M ethanol in ACSF for a 30 min period. One hour later, the ethanol perfusion was repeated. To test the functional integrity of the microdialysis probe in situ, each microdialysis session was completed with recording the effect of a 10–20 min perfusion of 500 μM N-methyl-d-aspartate (NMDA). The extracellular concentration profile of ethanol during intrahippocampal microdialysis with 1 M ethanol was estimated in a separate study in anesthetized rats. The ethanol content was measured in tissue slices surrounding the probe with gas chromatography (GC), and the generated data were analyzed with a mathematical model for microdialysis to estimate the concentration of ethanol at the recording site.ResultsThe predominant effect of the first intrahippocampal microdialysis with ethanol was a decrease in firing rate in both pyramidal cells and interneurons. In contrast, such firing rate decrease did not develop during the second ethanol perfusion. Subsequent NMDA perfusion still induced robust changes in the electrical activity of the neurons. The estimated extracellular ethanol concentration at the recording site was 45–70 mM.ConclusionThis study revealed that hippocampal neurons have the ability to develop acute tolerance to a single exposure of clinically relevant concentrations of ethanol in behaving rats, without influences from the rest of the body.
