Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
Gene transfer into mammalian cells by use of a nanosecond pulsed laser-induced stress wave.
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DOI：
10.1364/ol.29.001227
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发表时间：
2004-06
期刊：
影响因子：
3.6
通讯作者：
M. Terakawa;M. Ogura;Shunichi Sato;H. Wakisaka;H. Ashida;M. Uenoyama;Y. Masaki;M. Obara
中科院分区：
文献类型：
--
作者：
M. Terakawa;M. Ogura;Shunichi Sato;H. Wakisaka;H. Ashida;M. Uenoyama;Y. Masaki;M. Obara
利用纳秒脉冲激光诱导应力波（LISW）将质粒DNA成功地传递到哺乳动物细胞中。用编码绿色荧光蛋白的质粒选择性转染暴露于LISW的细胞。研究还表明，短暂、温和的细胞加热（约43摄氏度）可有效提高转染效率。
Plasmid DNA has been successfully delivered to mammalian cells by applying a nanosecond pulsed laser-induced stress wave (LISW). Cells exposed to a LISW were selectively transfected with plasmids coding for green fluorescent protein. It was also shown that transient, mild cellular heating (approximately 43 degrees C) was effective in improving the transfection efficiency.