MOLECULAR-CLONING OF PEA MESSENGER-RNAS ENCODING A SHOOT-SPECIFIC POLYPEPTIDE AND LIGHT-INDUCED POLYPEPTIDES
MOLECULAR-CLONING OF PEA MESSENGER-RNAS ENCODING A SHOOT-SPECIFIC POLYPEPTIDE AND LIGHT-INDUCED POLYPEPTIDES
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DOI：
10.1007/bf01578590
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发表时间：
1983-01-01
影响因子：
5.1
通讯作者：
WESSELS, JGH
中科院分区：
文献类型：
--
作者：
DEVRIES, SC;HARMSEN, MC;WESSELS, JGH
本文描述了豌豆幼苗mRNA序列对应的cDNA的分子克隆，该序列编码芽特异性多肽、核酮糖1,5二磷酸羧化酶的小亚基和光捕获叶绿素a/b复合物的一个组分。利用光生根多体poly(A)RNA与汞化后的暗生根多体poly(A)RNA进行异源液体杂交，并进行巯基层析，富集光生根多体poly(A)RNA的基因序列。克隆的射击特异性序列通过杂交释放翻译产物的二维电泳分析进行鉴定。克隆的芽特异性序列对应于光生和暗生芽中均存在的850 nt的mRNA，并产生mr27500和等电点4.7的蛋白体外翻译产物。
The molecular cloning of cDNA corresponds to pea seedling mRNA sequences encoding a shoot-specific polypeptide, the small subunit of the ribulose 1,5 biphosphate carboxylase and a component of the light-harvesting chlorophyll a/b complex is described. cDNA prepared from polysomal poly(A)RNA of light-grown shoots was enriched for shoot-specific and light-induced sequences by heterologous liquid hybridization with mercurated polysomal poly(A)RNA of dark-grown roots, followed by sulfhydryl chromatography. Cloned shoot-specific sequences were identified by 2D electrophoretic analysis of hybrid release translation products. The cloned shoot-specific sequence corresponded to a mRNA of 850 nt present both in light-and dark-grown shoots, and produced anin vitrotranslation product of Mr27 500 and isoelectric point of 4.7.