An electrochemical biosensor based on Hairpin-DNA modified gold electrode for detection of DNA damage by a hybrid cancer drug intercalation
An electrochemical biosensor based on Hairpin-DNA modified gold electrode for detection of DNA damage by a hybrid cancer drug intercalation
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DOI：
10.1016/j.bios.2019.02.071
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发表时间：
2019-05-15
影响因子：
12.6
通讯作者：
Chumbimuni-Torres, Karin
中科院分区：
文献类型：
--
作者：
Untiveros, Katherine Lozano;da Silva, Emanuella Gomes;Chumbimuni-Torres, Karin
采用示差脉冲伏安法（DPV）研究了一种新型高效的检测抗癌化合物（7 ESTAC 01）与DNA相互作用引起的DNA损伤的电化学生物传感器。该生物传感器由共价连接到金电极（GE）表面的茎环DNA（SL-DNA）探针组成，所述探针与互补DNA链（cDNA）杂交以形成双链DNA（dsDNA）。基于DPV氧化GE表面的电活性核酸信号，电化学研究了7 ESTAC 01的相互作用和对DNA的损伤。结果，用还原的7 ESTAC 01测试SL-DNA/GE和dsDNA/GE，显示鸟嘌呤和腺嘌呤的伏安信号在7 ESTAC 01存在下增加。在最佳条件下，dsDNA/GE生物传感器在7 ESTAC 01存在下表现出优异的DPV响应。7 ESTAC 01与小牛胸腺DNA（ctDNA）之间的键合相互作用通过UV-Vis吸收光谱、动态模拟（进行以研究生理条件下的DNA结构）和分子对接来证实。理论结果表明，氢键和嵌入的DNA的小沟，涉及疏水相互作用的存在。
An efficient and new electrochemical biosensor for detection of DNA damage, induced by the interaction of the hybrid anti-cancer compound (7ESTAC01) with DNA, was studied by differential pulse voltammetry (DPV). The biosensor consists of a Stem-Loop DNA (SL-DNA) probe covalently attached to the gold electrode (GE) surface that hybridizes to a complementary DNA strand (cDNA) to form a double-stranded DNA (dsDNA). The interaction and DNA damage induced by 7ESTAC01 was electrochemically studied based on the oxidation signals of the electroactive nucleic acids on the surface of the GE by DPV. As a result, the SL-DNA/GE and dsDNA/GE were tested with the reduced 7ESTAC01, showing the voltammetric signal of guanine and adenine, increase in the presence of 7ESTAC01. Under optimum conditions, the dsDNA/GE biosensor exhibited excellent DPV response in the presence of 7ESTAC01. The bonding interaction between 7ESTAC01 and calf thymus DNA (ctDNA) was confirmed by UV-Vis absorption spectroscopy, dynamic simulations (performed to investigate the DNA structure under physiological conditions), and molecular docking. Theoretical results showed the presence of hydrogen bonding and intercalation in the minor groove of DNA, involving hydrophobic interactions.