Conjugating Peptides onto 1D Rodlike Bionanoparticles for Enhanced Activity against Gram-Negative Bacteria.
Conjugating Peptides onto 1D Rodlike Bionanoparticles for Enhanced Activity against Gram-Negative Bacteria.
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DOI：
10.1021/acs.nanolett.0c04516
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发表时间：
2021-02
期刊：
影响因子：
10.8
通讯作者：
Guocheng Xie;Sijia Gao;J. Ou;M. Zhu;Man Wu;Xiaoyan Ju;Zhuang Li;Ye Tian;Z. Niu
中科院分区：
文献类型：
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作者：
Guocheng Xie;Sijia Gao;J. Ou;M. Zhu;Man Wu;Xiaoyan Ju;Zhuang Li;Ye Tian;Z. Niu
革兰氏阴性细菌具有不可渗透的外膜，是许多无法治疗的感染的罪魁祸首。50多年来缺乏新的相关抗生素的开发增加了威胁。肽被认为是最有前途的抗生素替代品。然而，由于现有肽的活性还不能与当前抗生素的活性相比，因此迫切需要提高其抗菌效率。本文中，我们将肽缀合到一维杆状烟草花叶病毒（TMV）上。在对抗革兰氏阴性细菌方面，所获得的纳米颗粒（肽-TMV）上的肽比游离肽优越数百倍上级。通过对大肠杆菌的形态学和基因检测，揭示了肽-TMV施用后，与膜损伤相关的高渗透压和产生的活性氧导致大肠杆菌死亡。此外，肽-TMV引起生物膜相关基因的下调，抑制生物膜形成。这项工作为对抗革兰氏阴性菌相关感染铺平了道路。
Gram-negative bacteria, which possess an impermeable outer membrane, are responsible for many untreatable infections. The lack of development of new relevant antibiotics for over 50 years has increased threats. Peptides are regarded as the most promising alternatives to antibiotics. However, since the activities of existing peptides are not yet comparable to those of current antibiotics, there is an urgent need to improve their antibacterial efficiencies. Herein, we conjugate peptides onto one-dimensional rod-like tobacco mosaic virus (TMV). The peptides on the obtained nanoparticles (peptide-TMV) are hundreds of times superior to free peptides in combating Gram-negative bacteria. Through morphology and gene detection of Escherichia coli, it was revealed that following peptide-TMV application, the high osmotic pressure related to membrane damage and the generated reactive oxygen species cause Escherichia coli's death. In addition, peptide-TMV causes a downregulation of biofilm-related genes, inhibiting biofilm formation. This work paves the way to combat Gram-negative bacteria-related infection.