New models of experimental parotitis and parotid gland distension in rats.
New models of experimental parotitis and parotid gland distension in rats.
复制标题
大鼠实验性腮腺炎和腮腺扩张的新模型。
DOI：
10.1007/978-1-61779-561-9_9
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Iwata K.
中科院分区：
文献类型：
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作者：
Okada-Ogawa A;Shinoda M;Honda K;Iwata K.
腮腺注射完全弗氏佐剂(CFA)后第2天和第3天，大鼠对面部外侧皮肤机械刺激的逃避阈值明显降低。生理盐水组大鼠的机械逃逸阈值也略有下降。通过测量组织伊文思蓝渗出量来验证和定量腮腺炎症。治疗后72 h，注射CFA的大鼠腮腺组织中Evans‘s蓝浓度明显高于注射生理盐水的大鼠。腮腺注射CFA后第10天，伊文思蓝浓度恢复到对照水平。腮腺内注射辣椒素不改变三叉神经脊束内亚核与尾侧亚核之间过渡区(Vi/Vc)的神经元活动。相反，辣椒素可显著增加位于C1/C2浅层神经元的感受野大小、机械反应和冷反应。C1/C2神经元亚群对腮腺机械扩张有反应，而Vi/VC神经元对腮腺机械扩张无反应。
A significant reduction of the escape threshold to mechanical stimulation of the lateral facial skin was observed bilaterally at days 2 and 3 after unilateral complete Freund’s adjuvant (CFA) administration into parotid gland. A slight reduction of mechanical escape threshold was also observed in rats with saline administration. The parotid gland inflammation was verified and quantified by measuring the tissue Evans’ blue dye extravasation. The Evans’ blue concentration in the parotid gland tissues was significantly greater in the CFA-injected rats than that of the saline-injected rats at 72 h after treatment. On day 10 after CFA administration into the parotid gland, the Evans’ blue concentration was recovered to the control level. The administration of capsaicin into the parotid gland did not alter neuronal activities in the transition zone between the trigeminal spinal subnucleus interpolaris and caudalis (Vi/Vc). In contrast, capsaicin administration induced significant increases in the receptive field size and mechanical and cold responses of neurons located in superficial laminae of the C1/C2. The subgroup of C1/C2 neurons responded to mechanical distension of the parotid gland, whereas no Vi/Vc neurons responded to parotid distension.