Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum.
Vasorin is a potential serum biomarker and drug target of hepatocarcinoma screened by subtractive-EMSA-SELEX to clinic patient serum.
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通过减法-EMSA-SELEX对临床患者血清筛选发现Vasorin是肝癌潜在的血清生物标志物和药物靶点。
DOI：
10.18632/oncotarget.3541
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发表时间：
2015-04-30
期刊：
影响因子：
--
通讯作者：
Shao N
中科院分区：
文献类型：
--
作者：
Li S;Li H;Yang X;Wang W;Huang A;Li J;Qin X;Li F;Lu G;Ding H;Su X;Hou L;Xia W;Shi M;Zhang H;Zhao Q;Dong J;Ge X;Sun L;Bai C;Wang C;Shen X;Fang T;Wang F;Zhang H;Shao N
我们报告了一种新的肝癌生物标志物，血管紧张素（Vasorin），筛选减EMSA-SELEX策略从AFP阴性的肝细胞癌（HCC）肝外转移患者的血清。100例肝癌患者血清中VEGFR-mRNA呈高表达，97例正常人和129例肝炎患者血清中VEGFR-mRNA呈高表达。经Q-PCR、IFA和Western blot进一步验证，肝癌细胞系和肝癌组织中VEGFRmRNA和蛋白水平的表达均高于正常对照组。RNA干扰和强制过表达实验证实，VEGFR 2促进细胞增殖和迁移，抑制细胞凋亡。microRNA miR 145和miR 146 a的下调是导致VEGF-C高表达的重要机制。结论：作为一种膜蛋白和/或游离蛋白，VEGFR 2可能是肝癌生物治疗的有效靶点，也是HCC诊断的潜在生物标志物。靶向VEGFR 1的小分子核苷酸是一种很有前途的肝癌生物治疗方法。
We report a new biomarker of hepatocarcinoma, vasorin (VASN), screened by a subtractive EMSA-SELEX strategy from AFP negative serum of hepatocellular carcinoma (HCC) patients with extrahepatic metastases. VASN was verified to be highly expressed in sera of 100 cases of HCC patients compared with 97 cases of normal persons and 129 cases of hepatitis patients. Further validation by Q-PCR, IFA and Western blot showed higher expression of VASN at mRNA and protein levels in HCC cell lines and HCC tissues than in normal controls. RNA interference and forced overexpression assays verified that VASN promotes cell proliferation and migration and inhibits apoptosis. Down-regulation of microRNA miR145 and miR146a is an important mechanism leading to high expression of VASN. Conclusion: As a membrane protein and/or as free protein, VASN may be an effective target for biological treatment of liver cancer and is a potential biomarker for HCC diagnosis. Small molecular nucleotides targeting VASN are promising biological therapies to HCC.
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