Nucleotide sequence of an insertion element, IS1.
Nucleotide sequence of an insertion element, IS1.
复制标题
插入元件 IS1 的核苷酸序列。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
E. Ohtsubo
中科院分区：
文献类型：
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作者：
H. Ohtsubo;E. Ohtsubo
PSM2、PSM1和PSM15是从插入序列IS1的两端自发缺失的R100衍生的小质粒。通过与EcoR1、HindIII、Hae III、Hpa II、Hha I、Hinf和AIu I的酶切，鉴定了与IS1相邻的区域以及IS1 DNA本身。IS1末端的大约30个碱基以相反的顺序重复。在IS1末端发生的缺失被发现是由于非法重组所致。基于重组热点周围的核苷酸序列，讨论了RNA聚合酶可能在这种重组现象中发挥重要作用的假设。
PSM2, PSM1, and PSM15 are small plasmids derived from R100 by spontaneous deletions at either end of the insertion sequence IS1. These plasmids were used to identify regions neighboring IS1 as well as the IS1 DNA itself, by cleavage with EcoR1, HindIII, Hae III, Hpa II, Hha I, Hinf, and AIu I. The nucleotide sequencing results demonstrate that IS1 contains 768 bases. About 30 bases at the ends of IS1 were found to be repeated in an inverted order. The deletions occurring at the ends of IS1 were found to be due to illegitimate recombination. The hypothesis that RNA polymerase could play an important role in such recombination phenomena is discussed based on the nucleotide sequences surrounding the recombinational hot spots.