Rapid Analysis of Perchlorate in Drinking Water at Parts per Billion Levels Using Microchip Electrophoresis
Rapid Analysis of Perchlorate in Drinking Water at Parts per Billion Levels Using Microchip Electrophoresis
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DOI：
10.1021/ac9029086
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发表时间：
2010-05-01
影响因子：
7.4
通讯作者：
Henry, Charles S.
中科院分区：
文献类型：
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作者：
Gertsch, Jana C.;Noblitt, Scott D.;Henry, Charles S.
建立了微芯片毛细管电泳（MCE）测定饮用水中高氯酸盐的方法。美国环境保护署（USEPA）最近提出了饮用水中高氯酸盐的健康咨询限值为十亿分之十五（ppb），这一水平需要大型复杂的仪器，如离子色谱-质谱联用仪（IC-MS）进行检测。一个便宜的，便携式的系统是需要日常在线监测应用程序的高氯酸盐在饮用水。本文提出了一种接触式电导检测高氯酸盐的MCE方法。该方法具有几个优点，包括相对于IC减少分析时间，固有的便携性，高选择性和最小的样品预处理。使用两性离子磺基甜菜碱表面活性剂、N-十六烷基-N，N-二甲基-3-铵基-1-丙磺酸盐（HDAPS）和N-十四烷基-N，N-二甲基-3-铵基-1-丙磺酸盐（TDAPS）实现了高氯酸盐与更丰富离子的分离。系统的性能和优化的分离化学，包括使用这些表面活性剂来解决高氯酸盐从其他阴离子，在这项工作中进行了讨论。该系统在标准品中的检测限为3.4 +/- 1.8 ppb（n = 6），在饮用水中的检测限为5.6 +/- 1.7 ppb（n = 6）。
A microchip capillary electrophoresis (MCE) system has been developed for the determination of perchlorate in drinking water. The United States Environmental Protection Agency (USEPA) recently proposed a health advisory limit for perchlorate in drinking water of 15 parts per billion (ppb), a level requiring large, sophisticated instrumentation, such as ion chromatography coupled with mass spectrometry (IC-MS), for detection. An inexpensive, portable system is desired for routine online monitoring applications of perchlorate in drinking water. Here, we present an MCE method using contact conductivity detection for perchlorate determination. The method has several advantages, including reduced analysis times relative to IC, inherent portability, high selectivity, and minimal sample pretreatment. Resolution of perchlorate from more abundant ions was achieved using zwitterionic, sulfobetaine surfactants, N-hexadecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (HDAPS) and N-tetradecyl-N,N-dimethyl-3-ammonio-1-propane sulfonate (TDAPS). The system performance and the optimization of the separation chemistry, including the use of these surfactants to resolve perchlorate from other anions, are discussed in this work. The system is capable of detection limits of 3.4 +/- 1.8 ppb (n = 6) in standards and 5.6 +/- 1.7 ppb (n = 6) in drinking water.