Roles for the two 1-butanol dehydrogenases of Pseudomonas butanovora in butane and 1-butanol metabolism.
Roles for the two 1-butanol dehydrogenases of Pseudomonas butanovora in butane and 1-butanol metabolism.
复制标题
丁香假单胞菌的两种 1-丁醇脱氢酶在丁烷和 1-丁醇代谢中的作用。
DOI：
10.1128/jb.184.16.4343-4350.2002
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发表时间：
2002
影响因子：
3.2
通讯作者：
Arp,DanielJ
中科院分区：
文献类型：
--
作者：
Vangnai,AlisaS;Sayavedra-Soto,LuisA;Arp,DanielJ
在丁烷或 1-丁醇上生长的丁醇假单胞菌表达两种 1-丁醇脱氢酶，一种醌蛋白 (BOH) 和一种醌血红素蛋白 (BDH)。 BOH 对 1-丁醇表现出高亲和力 (Km= 1.7 ± 0.2 μM)。 BOH 还氧化丁醛和 2-丁醇（Km 分别为 369 ± 85 μM 和 Km= 662 ± 98 μM）。 BOH 和 BDH 在三种不同水平的 1-丁醇（无毒水平 (0.1 mM)、生长支持水平 (2 mM) 和毒性水平 (40 mM)）下的 mRNA 诱导谱相似。当细胞在存在不同水平 1-丁醇的情况下在含柠檬酸盐的培养基中生长时，野生型 P. butanovora 可以耐受比 P 更高水平的 1-丁醇。 butanovora boh::tetstrain 和 theP。 Butanovora bdh::kanstrain。提出了一个模型，其中来自 1-丁醇氧化的电子遵循支化电子传输链。 BOH 可以与泛醌偶联，电子被传输至氰化物敏感的末端氧化酶。相反，来自 BDH 的电子可能会转移到对氰化物不太敏感的末端氧化酶。前一种途径可能主要在能量产生中发挥作用，而后者可能在 1-丁醇的解毒中更重要。
Pseudomonas butanovoragrown on butane or 1-butanol expresses two 1-butanol dehydrogenases, a quinoprotein (BOH) and a quinohemoprotein (BDH). BOH exhibited high affinity towards 1-butanol (Km= 1.7 ± 0.2 μM). BOH also oxidized butyraldehyde and 2-butanol (Km= 369 ± 85 μM andKm= 662 ± 98 μM, respectively). The mRNA induction profiles of BOH and BDH at three different levels of 1-butanol, a nontoxic level (0.1 mM), a growth-supporting level (2 mM), and a toxic level (40 mM), were similar. When cells were grown in citrate-containing medium in the presence of different levels of 1-butanol, wild-typeP. butanovoracould tolerate higher levels of 1-butanol than theP. butanovora boh::tetstrain and theP. butanovora bdh::kanstrain. A model is proposed in which the electrons from 1-butanol oxidation follow a branched electron transport chain. BOH may be coupled to ubiquinone, with the electrons being transported to a cyanide-sensitive terminal oxidase. In contrast, electrons from BDH may be transferred to a terminal oxidase that is less sensitive to cyanide. The former pathway may function primarily in energy generation, while the latter may be more important in the detoxification of 1-butanol.