Wide-field fluorescence lifetime imaging of cancer.
Wide-field fluorescence lifetime imaging of cancer.
复制标题
DOI：
10.1364/boe.1.000627
复制
发表时间：
2010-08-19
影响因子：
3.4
通讯作者：
Stamp GW
中科院分区：
文献类型：
--
作者：
McGinty J;Galletly NP;Dunsby C;Munro I;Elson DS;Requejo-Isidro J;Cohen P;Ahmad R;Forsyth A;Thillainayagam AV;Neil MA;French PM;Stamp GW
组织自体荧光的光学成像具有提供用于临床应用的表面肿瘤的快速无标记筛选和检测的潜力，包括当与内窥镜结合时。基于强度的对比度的定量成像是非常困难的，并且光谱分辨成像并不总是提供足够的对比度。我们证明，荧光寿命成像（FLIM）应用于内在组织自体荧光可以直接对比一系列的表面组织肿瘤，包括在胃肠道组织，使用紧凑的，临床上可部署的仪器实现前所未有的清晰度的宽视场荧光寿命图像。对于在355 nm处激发的FLIM，在癌性和健康结肠组织之间观察到统计学显著的对比。为了说明临床潜力，已经以接近视频速率采集了未染色的离体组织的宽场荧光寿命图像，这是朝着用于诊断和手术成像的实时FLIM迈出的重要一步，包括用于筛查和图像引导活检应用。
Optical imaging of tissue autofluorescence has the potential to provide rapid label-free screening and detection of surface tumors for clinical applications, including when combined with endoscopy. Quantitative imaging of intensity-based contrast is notoriously difficult and spectrally resolved imaging does not always provide sufficient contrast. We demonstrate that fluorescence lifetime imaging (FLIM) applied to intrinsic tissue autofluorescence can directly contrast a range of surface tissue tumors, including in gastrointestinal tissues, using compact, clinically deployable instrumentation achieving wide-field fluorescence lifetime images of unprecedented clarity. Statistically significant contrast is observed between cancerous and healthy colon tissue for FLIM with excitation at 355 nm. To illustrate the clinical potential, wide-field fluorescence lifetime images of unstained ex vivo tissue have been acquired at near video rate, which is an important step towards real-time FLIM for diagnostic and interoperative imaging, including for screening and image-guided biopsy applications.