INTRACELLULAR RECEPTOR SORTING DURING ENDOCYTOSIS - COMPARATIVE IMMUNOELECTRON MICROSCOPY OF MULTIPLE RECEPTORS IN RAT-LIVER
INTRACELLULAR RECEPTOR SORTING DURING ENDOCYTOSIS - COMPARATIVE IMMUNOELECTRON MICROSCOPY OF MULTIPLE RECEPTORS IN RAT-LIVER
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DOI：
10.1016/0092-8674(84)90315-5
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
SCHWARTZ, AL
中科院分区：
文献类型：
--
作者：
GEUZE, HJ;SLOT, JW;SCHWARTZ, AL
使用双标记定量免疫电镜对大鼠肝脏超薄冰冻切片进行比较，比较脱唾液酸糖蛋白受体 (ASGP-R)、6-磷酸甘露糖配体 (MP-R) 和聚合 IgA (IgA-R) 的内吞途径。所有 3 个蛋白均在高尔基复合体中、沿着包被小凹和囊泡的整个质膜以及受体和配体解偶联 (CURL) 的隔室内发现。受体随机出现在细胞表面、有涂层的凹坑和囊泡中。在 CURL 小管内，ASGP-R 和 MP-R 共定位，但 IgA-R 和 ASGP-R 显示出显着的微观异质性。因此，除了其在将再循环受体与配体解偶联和分选中的作用之外，CURL还充当将再循环受体（例如，ASGP-R）与参与转胞吞作用的受体（例如，IgA-R）分离的隔室。
Using double-label quantitative immunoelectron microscopy on ultrathin cryosections of rat liver, the endocytotic pathways of the receptors for asialoglycoprotein (ASGP-R), mannose-6-phosphate ligands (MP-R) and polymeric IgA (IgA-R), were compared. All 3 were found within the Golgi complex, along the entire plasma membrane in coated pits and vesicles, and within a compartment of uncoupling of receptors and ligand (CURL). The receptors occurred randomly at the cell surface, in coated pits and vesicles. Within CURL tubules ASGP-R and MP-R were colocalized, but IgA-R and ASGP-R displayed dramatic microheterogeneity. Thus, in addition to its role in uncoupling and sorting recycling receptor from ligand, CURL serves as a compartment to segregate recycling receptor (e.g., ASGP-R) from receptor involved in transcytosis (e.g., IgA-R).