An ABA-serotonin module regulates root suberization and salinity tolerance.
An ABA-serotonin module regulates root suberization and salinity tolerance.
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DOI：
10.1111/nph.18397
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发表时间：
2022-07
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影响因子：
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通讯作者：
Hai-Ping Lu;Qing Gao;Jian-Pu Han;Xiao-Hao Guo;Qing Wang;I. Altosaar;Marie Barberon;Jian-Xiang Liu;A. Gatehouse;Q. Shu
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作者：
Hai-Ping Lu;Qing Gao;Jian-Pu Han;Xiao-Hao Guo;Qing Wang;I. Altosaar;Marie Barberon;Jian-Xiang Liu;A. Gatehouse;Q. Shu
根中的木栓质充当物理屏障，防止水/矿物质损失。在拟南芥中，根系木栓化受到脱落酸（ABA）和乙烯的调节，以响应营养胁迫。 ABA 还介导微生物群和根内皮层之间矿物质营养稳态的协调。然而，尚不清楚这种调节系统是否对一般植物来说是常见的，以及是否涉及其他关键分子。我们发现，5-羟色胺在 ABA 下游发挥作用，调节水稻和拟南芥的木栓化，并负向调节水稻根部的木栓化，以响应盐度。我们发现 ABA 抑制血清素生物合成中关键基因 (OsT5H) 的转录，从而促进水稻根部木栓化。相反，OsT5H 的过度表达或补充外源血清素会抑制木栓化并降低对盐胁迫的耐受性。这些结果确定了控制水稻和拟南芥根系木栓化的 ABA-5-羟色胺调节模块，这可能代表了一种普遍机制，因为 ABA 和 5-羟色胺在植物中普遍存在。这些发现对于培育能够抵抗非生物胁迫的新作物品种以及制定生产富含木栓质根部以封存更多二氧化碳的策略具有重要意义，从而有助于减轻气候变化的影响。
Suberin in roots acts as a physical barrier preventing water/mineral losses. In Arabidopsis, root suberization is regulated by abscisic acid (ABA) and ethylene in response to nutrient stresses. ABA also mediates coordination between microbiota and root endodermis in mineral nutrient homeostasis. However, it is not known whether this regulatory system is common to plants in general, and whether there are other key molecule(s) involved. We show that serotonin acts downstream of ABA in regulating suberization in rice and Arabidopsis and negatively regulates suberization in rice roots in response to salinity. We show that ABA represses transcription of the key gene (OsT5H) in serotonin biosynthesis, thus promoting root suberization in rice. Conversely, overexpression of OsT5H or supplementation with exogenous serotonin represses suberization and reduces tolerance to salt stress. These results identify an ABA-serotonin regulatory module controlling root suberization in rice and Arabidopsis, which is likely to represent a general mechanism since ABA and serotonin are ubiquitous in plants. These findings are of significant importance to breeding novel crop varieties that are resilient to abiotic stresses and developing strategies for production of suberin-rich roots to sequestrate more CO2 , helping mitigate effects of climate change.