Hybrid nanoparticles with CO2-responsive shells and fluorescence-labelled magnetic cores
Hybrid nanoparticles with CO2-responsive shells and fluorescence-labelled magnetic cores
复制标题
具有 CO2 响应壳和荧光标记磁芯的混合纳米颗粒
DOI：
10.1039/c3tb21264e
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发表时间：
2014-01-01
影响因子：
7
通讯作者：
Yuan, Jinying
中科院分区：
文献类型：
--
作者：
Guo, Jun;Wang, Niejun;Yuan, Jinying
采用原子转移自由基聚合法，以SiO2包覆的染料标记的氧化铁纳米粒子（Fe3O4@dye/SiO2）为原料，合成了CO2响应性高分子聚甲基丙烯酸N，N-二乙氨基乙酯修饰的磁性纳米粒子（Fe3O4@dye/SiO2-PDEAEMA）。TEM、FT-IR和TGA证实并量化了接枝聚合物刷。制备的磁性纳米粒子（MNP）的荧光标记，所示的荧光光谱和荧光显微镜图像。这表明纳米颗粒可以用作成像探针来监测生物细胞或其他系统的运动。MNP表面的PDEAEMA聚合物刷赋予其蛋白质吸附能力。该聚合物也是CO2响应性的，因此它是一种CO2触发的可逆的蛋白质吸附/释放材料，这是由牛血清白蛋白（BSA）吸附实验证明。结果表明，交替通入CO2和N2-容易实现蛋白质的可逆吸附/释放。
CO2-responsive polymer poly(N,N-diethylaminoethylmethacrylate)-modified magnetic nanoparticles (Fe3O4@dye/SiO2-PDEAEMA) were synthesized by atom transfer radical polymerization from the surfaces of silica coated, dye-labelled iron oxide nanoparticles (Fe3O4@dye/SiO2). TEM, FT-IR, and TGA confirmed and quantified the grafted polymer brushes. The prepared magnetic nanoparticles (MNPs) were fluorescence labelled, as shown by the fluorescence spectra and fluorescence microscopy images. This indicated that the nanoparticles could be utilized as imaging probes to monitor the movement of biological cells or other systems. The PDEAEMA polymer brushes on the surface of MNPs endowed them with a protein adsorption ability. The polymer was also CO2-responsive, so it was a CO2-triggered reversible protein adsorption/release material, which was demonstrated by the bovine serum albumin (BSA) adsorption experiments. The results indicated that the reversible adsorption/release of protein by bubbling CO2 and N-2 alternately was easy to achieve.