Isolation and characterization of proteins associated with the lung surfactant system.
Isolation and characterization of proteins associated with the lung surfactant system.
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与肺表面活性剂系统相关的蛋白质的分离和表征。
DOI：
10.1080/00327487708065510
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
John U. Balls
中科院分区：
文献类型：
--
作者：
John J. Maguire;Sue A. Shelley;June E. Paclga;John U. Balls
兔肺洗涤液和纯化的肺表面活性剂被脱脂，而没有沉淀或蛋白质损失。这使得通过电泳和免疫电泳技术能够有效地研究蛋白质。肺冲洗液含有分泌型免疫球蛋白A和几种血清蛋白。纯化的肺表面活性物质的蛋白质组成与未分级的肺洗涤液相同，证实了我们先前的研究，该研究表明，通过用等渗盐水进行肺泡灌洗获得的表面活性物质磷脂没有特异性蛋白质。
Rabbit lung washings and purified lung surfactant were delipidated without precipitation or loss of protein. This enabled effective study of the proteins by electrophoretic and immunoelectrophoretic techniques. The lung washings contained secretory immunoglobulin A and several serum proteins. The protein composition of purified lung surfactant was the same as the unfractionated lung washings confirming our previous study which indicated that there is no specific protein associated with surfactant phospholipids obtained by alveolar lavage with isotonic saline.