Evaluation of swollenin from Trichoderma pseudokoningii as a potential synergistic factor in the enzymatic hydrolysis of cellulose with low cellulase loadings
Evaluation of swollenin from Trichoderma pseudokoningii as a potential synergistic factor in the enzymatic hydrolysis of cellulose with low cellulase loadings
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DOI：
10.1007/s11274-011-0650-5
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发表时间：
2011-08-01
影响因子：
4.1
通讯作者：
Liu, Weifeng
中科院分区：
文献类型：
--
作者：
Zhou, Qingxin;Lv, Xinxing;Liu, Weifeng
Swollenin是一种新的植物膨胀素蛋白，已被认为具有破坏纤维素的活性。在本研究中，利用黑曲霉对拟康氏木霉S38菌株的重组溶胀素进行了生产和纯化，最终从1 L发酵上清液中获得了10 mg以上的纯化蛋白。重组蛋白在处理纤维素时表现出很低的内切葡聚糖酶活性，并导致结晶度略有增加。SWO2与低剂量纤维素酶同时孵育，对纤维素酶的降解有显著的协同作用。具体来说，当纤维素酶降解纤维素后，用溶胀素对纤维素进行预处理，可以获得更大的协同活性。因此，我们的结果为溶胀素在纤维素材料的高效糖化中的潜在应用提供了一种新的途径。
Swollenin is a novel plant expansin-like protein that has been proposed to have a cellulose disruption activity. In this study, the recombinant swollenin (SWO2) from Trichoderma pseudokoningii S38 was successfully produced and purified in Aspergillus niger with a final yield of up to 10 mg of purified protein from 1 l of fermentation supernatant. The recombinant protein was found to exhibit very low level of endoglucanase activity and caused a slight increase in the crystallinity when treating cellulose. Simultaneous incubation of SWO2 with low-dose cellulases resulted in a significant synergistic activity in cellulose hydrolysis. Specifically, an even greater increase in the synergistic activity was obtained when cellulose was pretreated with swollenin followed by cellulase hydrolysis. Our results, therefore, provide a novel approach for the potential application of swollenin in the efficient saccharification of cellulosic materials.