Differentiation and retrodifferentiation of U937 cells: reversible induction and suppression of intermediate filament protein synthesis.
Differentiation and retrodifferentiation of U937 cells: reversible induction and suppression of intermediate filament protein synthesis.
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发表时间：
1990-04
影响因子：
6.6
通讯作者：
R. Hass;G. Giese;G. Meyer;A. Hartmann;T. Dörk;L. Köhler;K. Resch;P. Traub;M. Goppelt-Strübe
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文献类型：
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作者：
R. Hass;G. Giese;G. Meyer;A. Hartmann;T. Dörk;L. Köhler;K. Resch;P. Traub;M. Goppelt-Strübe
用佛波醇酯12-O-十四烷酰佛波醇-13-乙酸酯（TPA）诱导人U937肿瘤细胞分化72 h，观察到细胞形态和功能的显著变化。这些包括细胞相互粘附和粘附到基质，细胞表面抗原表达的改变和自主增殖的停止。在这项研究中，我们表明，RNA杂交分析和免疫印迹的增强表达的中间丝（IF）亚基蛋白波形蛋白，核纤层蛋白A和核纤层蛋白C伴随TPA诱导的分化过程。然而，在TPA不存在下分化的U937细胞的长期培养（超过28天）后，贴壁细胞缩回它们的伪足，脱离并再次开始增殖。这种“逆分化”过程，以前没有描述过，通过IF mRNA和蛋白质合成的快速下调回到未分化的U937对照细胞的水平来实现。这些数据表明波形蛋白和核纤层蛋白A和C的表达与这些细胞中发生的分化过程之间的功能关系。
Significant morphological and functional changes were observed when human monoblastoid U937 tumor cells growing in suspension were induced by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) for 72 h to differentiate along the monocyte/macrophage pathway. These include adherence of the cells to each other and to the substratum, alterations in cell-surface antigen expression and cessation of autonomous proliferation. In this study, we show by both, hybridization analysis of RNA and immunoblotting that an enhanced expression of the intermediate filament (IF) subunit proteins vimentin, lamin A and lamin C accompanied the TPA-induced differentiation process. After long-term culture of differentiated U937 cells in the absence of TPA (more than 28 days), however, the adherent cells retracted their pseudopodia, detached and started again to proliferate. This "retrodifferentiation" process, not previously described was paralleled by a rapid down-regulation of both, IF mRNA and protein synthesis back to the level of undifferentiated U937 control cells. These data suggest a functional relationship between the expression of vimentin and lamins A and C and the differentiation process taking place in these cells.