Serial FNA allows direct sampling of malignant and infiltrating immune cells in patients with B-cell lymphoma receiving immunotherapy.
Serial FNA allows direct sampling of malignant and infiltrating immune cells in patients with B-cell lymphoma receiving immunotherapy.
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DOI：
10.1002/cncy.22531
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发表时间：
2022-03
影响因子：
3.4
通讯作者：
Long SR
中科院分区：
文献类型：
--
作者：
Mooney KL;Czerwinski DK;Shree T;Frank MJ;Haebe S;Martin BA;Testa S;Levy R;Long SR
细针抽吸 (FNA) 用于诊断恶性肿瘤、复发和转移。该过程快速、耐受性良好，并且可以通过超声引导来促进。在这里，我们描述了在低度淋巴瘤患者中通过原位疫苗接种进行免疫治疗的四项临床试验中使用系列 FNA 收集细胞材料的经验。从 44 名患者身上收集了 296 个 FNA 样本，每位患者的时间跨度约为六周。样品的数量和质量足以通过流式细胞术和/或单细胞 mRNA 测序进行分析。 FNA 样本平均产生 12 x 106 个细胞，平均细胞活力为 86%。与匹配的外周血样本相比，从肿瘤淋巴结收集的材料在细胞群的比例和表型方面存在显着差异。将直接从患者获得的 FNA 获得的流式细胞术结果与离体进行的 FNA 以及手术切除后同一淋巴结的分离进行比较，证实患者的 FNA 采样准确地代表了肿瘤和微环境。对经过免疫治疗的目标淋巴结与未经治疗的肿瘤部位的淋巴结的 FNA 样本进行分析，可以深入了解特定免疫治疗方案的影响。这是描述在免疫治疗临床试验期间使用连续 FNA 采样来收获细胞材料的最大规模的研究。这项技术的成功为 FNA 采样打开了大门，以显着扩大未来对研究药物动态效应的研究，无论是免疫疗法还是靶向疗法。细针抽吸 (FNA) 是一种在免疫治疗方案过程中直接从多个肿瘤结节连续采集样本的可靠方法。然后可以对这些细胞样本进行研究和分析，以跟踪患者对治疗的反应，并研究肿瘤及其微环境。
Fine needle aspiration (FNA) is used to diagnose malignancies, recurrences, and metastases. The procedure is quick, well-tolerated, and can be facilitated by ultrasound guidance. Here we describe our experience using serial FNAs to harvest cellular material during four clinical trials of immunotherapy by in situ vaccination in patients with low grade lymphoma. Two hundred and ninety-six FNA samples were collected from 44 patients spanning approximately six weeks for each patient. Samples were sufficient in quantity and quality to be analyzed by flow cytometry and/or single-cell mRNA sequencing. FNA samples yielded an average of 12 x 106 cells with a mean cellular viability of 86%. Material collected from the tumor lymph nodes differed significantly in proportions and phenotype of cellular populations compared to matched peripheral blood samples. Comparison of flow cytometry results obtained by FNA directly from the patient to that of an FNA performed ex vivo and a dissociation of the same lymph node after surgical excision confirmed that FNA sampling of the patient accurately represents the tumor and the microenvironment. Analysis of the FNA samples from immunotherapy-treated target lymph nodes vs nodes from non-treated tumor sites provided insight into the impact of specific immunotherapy regimens. This is the largest study describing the use of serial FNA sampling to harvest cellular material during immunotherapy clinical trials. The success of this technique opens the door for FNA sampling to expand significantly future investigation of the dynamic effects of investigational agents, be they immune therapies or targeted therapies. Fine needle aspiration (FNA) is a dependable method of collecting samples directly from multiple tumor nodules sequentially over the course of immunotherapy regimes. These cellular samples can then be studied and profiled to track patient responses to therapy, and to study both the tumor and its microenvironment.
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