Diagnostic value of GLUT-1 immunoreactivity to distinguish benign from malignant cystic squamous lesions of the head and neck in fine-needle aspiration biopsy material
Diagnostic value of GLUT-1 immunoreactivity to distinguish benign from malignant cystic squamous lesions of the head and neck in fine-needle aspiration biopsy material
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DOI：
10.1002/dc.20127
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发表时间：
2004-11-01
影响因子：
1.3
通讯作者：
De Las Casas, LE
中科院分区：
文献类型：
--
作者：
Weiner, MF;Miranda, RN;De Las Casas, LE
头颈部囊性鳞状细胞癌(SCC)与良性囊性鳞状细胞病变(BCSL)的区别在细针吸取活检(FNAB)材料上可能存在问题，特别是当BCSL显示上皮反应性异型性或SCC分化良好时。葡萄糖转运蛋白1(GLUT-1)是一种促进细胞表面葡萄糖转运的蛋白，在口腔和下咽鳞状细胞癌等多种肿瘤中均有异常表达。我们通过免疫组织化学方法评估GLUT-1在头颈部囊性鳞状细胞癌和BCSL中的表达，以确定其在头颈部囊性鳞癌鉴别中的价值。我们对所有头颈部囊性鳞状细胞癌标本进行了5年的回顾性分析。取24个囊性鳞状细胞病变的细胞块材料，其中8个(33%)BCSL(7个鳃裂囊肿和1个甲状舌管囊肿[TDC])和16个(67%)转移性鳞状细胞癌囊性/液化变性，并用抗GLUT-1免疫组织化学染色。当至少10%的鳞状细胞表现出明显的细胞膜反应时，GLUT-1的表达被认为是阳性的。所有16例鳞状细胞癌中均检测到GLUT-1阳性表达，而在8例BCSL中均未检测到GLUT-1阳性表达。在癌组织中，大多数恶性细胞呈GLUT-1活性，只有少数角化的鳞状珍珠状高分化SCC细胞不表达GLUT-1，细胞块材料中GLUT-1的表达有助于区分头颈部囊性SCC和BCSL。当细胞学结果不明确时，结合临床和放射学相关性，GLUT-1免疫反应可作为重要的诊断辅助手段。(C)2004年Wiley-Liss公司
The distinction of cystic squamous-cell carcinoma (SCC) from benign cystic squamous lesions (BCSLs) of the head and neck can be problematic on fine-needle aspiration biopsy (FNAB) material, particularly when BCSLs display epithelial reactive atypia or when SCC is well differentiated. Glucose transporter 1 (GLUT-1), a facilitative cell surface glucose transport protein, is aberrantly expressed in many cancers including oral and hypopharyngeal SCC. We evaluated the expression of GLUT-1 by immunochemistry on FNAB material to determine its value in distinguishing cystic SCC from BCSL of the head and neck.A 5-yr retrospective review of all head and neck cystic squamous lesions having FNAB specimens with cell block material, radiological studies, and histological confirmation was performed at our institution. Cell block material from 24 cystic squamous lesions, including 8 (33%) BCSL (7 branchial cleft cysts and 1 thyroglossal duct cyst[TDC]) and 16 (67%) metastatic SCCs with cystic/liquefactive degeneration, was retrieved and immunostained with anti-GLUT-1. GLUT-1 expression was considered positive when at least 10% of squamous cells exhibited distinct cell membrane reactivity.Positive GLUT-1 immunostaining was detected in all 16 SCCs and in none of the 8 BCSLs. In the carcinoma cases, the majority of malignant cells exhibited GLUT-1 reactivity; only a minor population of well-differentiated SCC cells displaying keratinization and arranged as squamous pearls did not express GLUT-1.GLUT-1 expression in cell block material can help to distinguish cystic SCCs from BCSLs of the head and neck. In conjunction with clinical and radiological correlation, GLUT-1 immunoreactivity can be an important diagnostic aid when the cytological findings are ambiguous. (C) 2004 Wiley-Liss, Inc.