Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background
Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background
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TUSMi003-A 诱导多能干细胞 (iPSC) 系的建立，源自一名具有 ApoE3/4 遗传背景的 62 岁中国汉族阿尔茨海默病患者
DOI：
10.1016/j.scr.2018.01.005
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发表时间：
2018
影响因子：
1.2
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Wang Ying;Chen Keni;Sun Heng;Lei Ying;Zhao Jian;Yu Hongxiang;Zhang Yue;Zhang Jing;Li Gang;Meng Shulin;Lei Y;Li G
一位62岁的中国汉族阿尔茨海默病（AD）女性患者，具有ApoE 3/4遗传背景，捐献了她的外周血单个核细胞（PBMC）。使用非整合附加型载体系统用人OKSM转录因子重编程PBMC。无转基因iPSC的多能性通过多能性标志物的免疫细胞化学和iPSC在体外自发分化成3个胚层的能力来证实。此外，iPSC系显示正常核型。我们的模型可能为进一步研究病理机制、识别早期生物标志物以及AD的药物测试研究提供了一个很好的平台。
A 62-year old Chinese Han Alzheimer's disease (AD) female patient with ApoE3/4 genetic background donated her Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogrammed PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layersin vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in AD.