Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1
Endogenous Indole Pyruvate Pathway for Tryptophan Metabolism Mediated by IL4I1
复制标题
IL4I1 介导的内源性吲哚丙酮酸途径色氨酸代谢
DOI：
10.1021/acs.jafc.0c03735
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发表时间：
2020-09-30
影响因子：
6.1
通讯作者：
Chen, Gang
中科院分区：
文献类型：
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作者：
Zhang, Xia;Gan, Min;Chen, Gang
L-色氨酸（Trp）广泛应用于食品和饲料执法，在生物过程中发挥重要作用。Trp的各种代谢产物发挥其强大的功能。吲哚丙酮酸途径是肠道微生物群中色氨酸代谢的主要途径之一，提供许多吲哚衍生物，其可以通过激活芳烃受体（AHR）信号通路来调节肠道稳态和粘膜免疫。在本研究中，我们构建了IL 4 I1过表达的293 T细胞系，发现IL 4 I1可以催化Tip产生吲哚-3-乙酸（IAA）和吲哚-3-甲醛（IAld）。此外，IAA和IAld都在树突状细胞（DCs）中积累，并且可以刺激CYP 1A 1的表达。我们的研究结果表明，吲哚丙酮酸途径在宿主细胞中的存在与IL 4 I1作为关键酶。IL 4 I1介导的Trp代谢暗示了饮食对免疫的影响。
L-tryptophan (Trp) is widely used in food and feed enforcement to play an important role in biological processes. Various metabolites of Trp perform its potent function. The indole pyruvate pathway is one of the main pathways of Trp metabolism in the gut microbiota, providing numerous indole-derivatives, which can modulate intestinal homeostasis and mucosal immunity by activating the aryl hydrocarbon receptor (AHR) signaling pathway. In this study, we constructed an IL4I1-overexpressed 293T cell line and found that IL4I1 can catalyze Tip to produce indole-3-acetic acid (IAA) and indole-3-carboxaldehyde (IAld). Moreover, both IAA and IAld are accumulated in dendritic cells (DCs) and can stimulate the expression of CYP1A1. Our results demonstrate the existence of the indole pyruvate pathway in host cells with IL4I1 as the key enzyme. The IL4I1-mediated Trp metabolism implies the role of dietary impact on immunity.