Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
复制标题
针对 FokI 核酸酶结构域的 DNA 适体的选择和表征
DOI：
10.1007/978-1-4939-8799-3_12
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发表时间：
2018
影响因子：
--
通讯作者：
Ikebukuro Kazunori
中科院分区：
文献类型：
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作者：
Nishio Maui;Yamagishi Ayana;Tsukakoshi Kaori;Kato Yoshio;Nakamura Chikashi;Ikebukuro Kazunori
使用位点特异性核酸酶（SSN）进行基因组编辑可能对基因治疗有效，因为SSN可以修饰靶基因。然而，基因组编辑在临床应用中的主要限制是细胞内过量SSN的脱靶效应。因此，SSN的受控递送系统是必要的。以前，我们已经报道了锌指核酸酶（ZFN）递送系统，其结合了针对FokI核酸酶结构域（FokI）的DNA适体和纳米针。在这里，我们描述了如何选择针对FokI的DNA适体并表征其用于基因组编辑应用。
Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells. Therefore, a controlled delivery system for SSNs is necessary. Previously we have reported on a zinc finger nuclease (ZFN) delivery system, which combined DNA aptamers against FokI nuclease domain (FokI) and nanoneedles. Here, we describe how DNA aptamers against FokI were selected and characterized for genome editing applications.