Spatial-temporal expression of NDRG2 in rat brain after focal cerebral ischemia and reperfusion
Spatial-temporal expression of NDRG2 in rat brain after focal cerebral ischemia and reperfusion
复制标题
局灶性脑缺血再灌注后大鼠脑内NDRG2的时空表达
DOI：
10.1016/j.brainres.2011.01.023
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发表时间：
2011-03-25
期刊：
影响因子：
2.9
通讯作者：
Xiong, Lize
中科院分区：
文献类型：
--
作者：
Li, Yan;Shen, Lan;Xiong, Lize
据报道，N-myc下游调节基因2（NDRG 2）在神经系统中广泛表达。然而，NDRG 2在局灶性脑缺血脑组织中的表达及其潜在作用尚不清楚。在此，我们研究了短暂局灶性脑缺血大鼠脑内NDRG 2的时空表达。雄性Sprague-Dawley大鼠经历了120分钟的大脑中动脉短暂闭塞。在再灌注后4、12、24和72 h处死大鼠并收获脑样品。采用逆转录聚合酶链反应（RT-PCR）、免疫印迹和免疫组织化学方法检测NDRG 2在脑组织中的表达。TUNEL法检测细胞凋亡。结果显示，NDRG 2表达于缺血半暗带星形胶质细胞样形态的细胞。缺血半暗带区NDRG 2 mRNA和蛋白表达于再灌注4 h开始增加，24 h达高峰。通过使用免疫荧光，NDRG 2信号与GFAP阳性星形胶质细胞共定位，并且在再灌注后24 h星形胶质细胞中的NDRG 2表达从细胞质易位到核定位。TUNEL和NDRG 2免疫荧光双染显示，部分NDRG 2信号与TUNEL阳性细胞共定位，且随着NDRG 2阳性信号的增强，凋亡细胞增多。总之，短暂性局灶性脑缺血后，NDRG 2表达在缺血半暗带上调。NDRG 2在脑卒中后星形胶质细胞中的表达可能在脑卒中后细胞凋亡中起重要作用。(C)2011 Elsevier B. V.保留所有权利。
N-myc downstream regulated gene 2 (NDRG2) was reported to be widely expressed in the nervous system. However, the expression and potential role of NDRG2 in focal cerebral ischemia brain remain unclear. Herein, we investigated spatial-temporal expression of NDRG2 in the rat brain following transient focal cerebral ischemia. Male Sprague-Dawley rats underwent a 120-min transient occlusion of middle cerebral artery. Rats were killed and brain samples were harvested at 4, 12, 24, and 72 h after reperfusion. Expression of NDRG2 in the brain was determined by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot analysis and immunohistochemical staining. Cellular apoptosis was assessed by TUNEL staining. The results showed that NDRG2 was expressed on cells with an astrocytes-like morphology in ischemic penumbra. NDRG2 mRNA and protein expression began to increase at 4 h after reperfusion and peaked at 24 h in the ischemic penumbra. By using immunofluorescence, NDRG2 signals were co-localized with GFAP-positive astrocytes, and NDRG2 expression in astrocytes translocated from a cytoplasm to a nuclear localization at 24 h after reperfusion. Double immunofiuorescent staining for TUNEL and NDRG2 showed that some NDRG2 signals co-localized with TUNEL-positive cells, and that the apoptotic cells increased with enhancement of NDRG2-positive signals. In conclusion, NDRG2 expression is up-regulated in ischemic penumbra following transient focal cerebral ischemia. NDRG2 expression in astrocytes may play important pathological roles in cell apoptosis after stroke. (C) 2011 Elsevier B.V. All rights reserved.