Design of porous S-doped carbon nanostructured electrode sensor for sensitive and selective detection of guanine from DNA samples
Design of porous S-doped carbon nanostructured electrode sensor for sensitive and selective detection of guanine from DNA samples
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DOI：
10.1016/j.micromeso.2021.111097
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发表时间：
2021-06
影响因子：
5.2
通讯作者：
Mohammed Y. Emran;M. Shenashen;S. El‐Safty;M. Selim
中科院分区：
文献类型：
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作者：
Mohammed Y. Emran;M. Shenashen;S. El‐Safty;M. Selim
电化学传感器/生物传感器的发展是一个有趣的研究课题，用于敏感和选择性的筛选生物分子的一些疾病的诊断。制备了微孔硫掺杂碳的活性界面，用于设计高灵敏度生物样品中选择性鸟嘌呤（GUA）检测的传感协议。基于S掺杂碳纳米颗粒（S-CNPs）的电极充当具有异质表面和多活性中心的高活性介体，使得能够与活性中心强结合、短距离路径、容易的电子/分子扩散和低表面电阻。具体地说，表面电极活性可能与活性掺杂的S原子到碳纳米球的形态，界面附近沿着与堆叠和非堆叠的颗粒，纳米/微米/介孔的凹槽，和表面的不均匀性与一些脊，孔状漩涡，和粗糙度。所设计的基于S-CNP的GUA传感器表现出快速响应信号，低检测限（0.0026 μmol L−1）和宽线性范围（0.005-0.5 μmol L−1）。的S-CNP感觉协议显示了一个高度敏感和选择性的GUA传感器从水解和DNA暴露于氧化应激释放。我们的研究结果表明，指定的S-CNP传感器可用于在各种生理条件下的DNA评估和人体健康质量的临床调查。
The development of electrochemical sensor/biosensor for the sensitive and selective screening of biomolecules is an interesting research topic for diagnosing some diseases. The active interfacial surface of microporous sulfur-doped (S-doped) carbon is fabricated to design sensory protocol for selective guanine (GUA) detection in biological samples with high sensitivity. S-doped carbon nanoparticles (S-CNPs) based electrode acts as a highly active mediator with heterogeneous surfaces and multi-active centers, enabling strong binding to active centers, short distance pathways, facile electron/molecular diffusion, and low surface resistance. Specifically, the surface electrode activities might be related to the actively-doped S atom onto carbon nanosphere morphology, interface vicinity along with stacked and non-stacked particles, nano-/micro-/meso-pores like grooves, and surface heterogeneity with a number of ridges, and holes-like vortexes, and roughness. The designed S-CNP-based sensor of GUA demonstrates fast-response signaling, low detection limit (0.0026 μmol L−1), and a wide linear range (0.005–0.5 μmol L−1). The S-CNP sensory protocol shows a highly sensitive and selective GUA sensor liberated from hydrolyzed and DNA exposed to oxidative stresses. Our finding indicates that the designated S-CNP sensor can be used in DNA-assessing at various physiological conditions and in clinical investigation of human health quality.